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Tizapa volcanogenic massive sulfide (VMS) deposit is hosted in greenschist facies metamorphic rocks; footwall is green schist of felsic to mafic metavolcanic rocks and hanging wall is graphite schist of metasedmentary pelitic rock. Pb-Pb dating of ore samples indicates 103.4Ma to 156.3Ma for the age of mineralization (JICA/MMAJ, 1991) .Hanging wall graphite schist is partially calcareous and overlaid by upper formations consisting of calcareous shale and limestone. δ 13 C(‰)PDB values were measured for carbonate and organic material in the graphite schist, and δ 18 O(‰)SMOW values were also measured for same carbonate. Although carbonate and organic material were affected by the metamorphism after mineralization, δ 18 O of carbonate decreases from +22.1‰ to +17.9‰, δ 13 C of carbonate decreases from +0.8‰ to -4.3‰ and δ 13 C of organic material decreases from -10.0‰ to -15.6‰ with the decrease of vertical distances to ore deposit from 54.20m to 10.28m. This phenomenon might indicate the change of sedimentary environment of the reduced condition where the sulfides were precipitated, with the change of temperature. If this assumption is adequate, the method applied in this study is useful for semi-quantitative evaluation to distinguish the favorable condition for the precipitation of sulfides of VMS.