A Leucose Enzoótica dos Bovinos (LEB) é uma doença causada por um retrovírus, com casos notificados no mundo todo e que se alastra progressivamente pelos rebanhos determinando grandes prejuízos à bovinocultura brasileira. O objetivo do presente trabalho foi determinar a soroprevalência da LEB nos bovinos abatidos no matadouro frigorífico do município de Anchieta, estado do Espírito Santo. Para isso foram coletadas 933 amostras séricas, colhidas na linha de abate do frigorífico durante o mês de novembro de 2013, foram examinados pelo Teste de Imunodifusão em Ágar Gel (IDGA) para a detecção dos anticorpos específicos contra o Vírus da Leucose Bovina (VLB). O teste foi realizado seguindo instruções do fabricante Tecpar ®, onde se identificou uma prevalência de 21,86%, sendo 204 das amostras coletadas soropositivas para a LEB. A prevalência encontrada no presente trabalho é menor que a de 39,8% definida por Birgel Júnior et al., ( 2006) como média da região sudeste. Porém, a prevalência encontrada é bem expressiva, mostrando que a LEB já se encontra difundida no rebanho bovino de corte do estado, e que tem totais condições de trazer grandes prejuízos à bovinocultura do Estado. Palavras chaves: Epidemiologia, linfoma, leucemia bovina Occurrence of leukemia virus in cattle slaughtered beneath the inspection in the State of Espírito Santo in 2013ABSTRACT. The Enzootic Bovine Leukosis (LEB) is a disease caused by a retrovirus, with cases reported in all the world and gradually spreads determining losses to Brazilian cattle. The objective of the study is defining the seroprevalence of LEB in cattle slaughtered in the city of Anchieta, Espírito Santo. 933 serum samples was collected in the slaughter line during the month of November 2013. The samples were examined by the immunodiffusion test in agar gel (IDGA) for the detection of specific antibodies against bovine leukosis virus (VLB). The test was performed following manufacturer's instructions Tecpar ®. The prevalence detected was 21.86 %, 204 samples was soropositive for the LEB. The prevalence found in this study is lower than 39.8% found by Birgel Junior et al., (2006) with is the media of the southeast region. However, the prevalence is very expressive, showing that LEB is already widespread in the beef cattle in Espírito Santo, and that has total conditions to bring considerable losses to cattle in the state.

The objective of this study was to investigate the in vitro action of triiodothyronine (T3) on the chondrogenic differentiation of adipose tissue-derived stem cells (ASCs) of female rats, with different time periods and doses. ASCs were extracted from female Wistar rats and were cultured in chondrogenic medium with and without the presence of T3. Five groups were established: 1) ASCs without T3; and 2,3,4,5) ASCs with 0.01, 1, 100 and 1,000 nM T3, respectively). After 7, 14 and 21 days, cell morphology, chondrogenic matrix formation, and expression of Sox9, aggrecan, collagen II, and collagen X were evaluated. The Student-Newman-Keuls test was used. ASCs showed CD54, CD73, and CD90 before chondrogenic differentiation. The hormone treatment did not alter chondrogenic matrix formation, Sox9 expression at 14 or 21 days, or expression of collagen II or collagen X at any time. However, the 0.01, 1, and 1000 nM T3 doses decreased Sox9 expression at 7 days. In conclusion, chondrogenic differentiation of ASCs of female rats is not influenced by T3.