The objectives of this study were: i) to isolate Listeria spp. and Listeria monocytogenes in broiler wing meat samples, ii) to confirm the isolates by PCR, based on prs and hly A gene sequences, iii) to determine the seasonal and monthly distribution of the isolates. A total of 120 broiler wing meat samples (60 packaged pieces wrapped using strech film in styrofoam plates and 60 unpackaged pieces) bought from different markets in Hatay province were analysed. Listeria spp. was isolated from 57 (47.5%) out of 120 samples. Fifty-four, out of 57 Listeria spp. isolates were identified as L. monocytogenes. L. monocytogenes was isolated from the samples collected during the spring, winter, summer, and autumn at the levels of 26.6%, 40%, 53.3%, 60%, respectively. In this study, the isolation rates were found to be the highest in autumn, while the isolation rates were found to be the lowest in spring. As a consequence, high prevalence of Listeria spp. and L. monocytogenes in poultry wing meat samples may pose a risk for human health. We consider that with obeying the rules of good hygiene practices (GHP), good manufacturing practices (GMP) and HACCP can minimize the contamination with Listeria spp.Keywords: broiler wing meat; L. monocytogenes; Listeria spp.; hygienic quality.Practical Application: Taking hygienic measures in broiler flocks at farm level may minimize the contamination with Listeria.
In this study, the prevalence and distribution of various Arcobacter spp. were investigated in samples taken from the cloacae of healthy domestic geese raised in Turkey. A membrane filtration technique with a non-selective blood agar was employed after enrichment in Arcobacter enrichment broth (AEB) to isolate a wide range of Arcobacter spp. In addition, the isolates were characterized phenotypically and identified at species level using a multiplex-PCR assay. A total of 90 cloacal swab samples taken from geese, collected on three farms (18, 25, 47 samples, respectively), were examined. Of the samples examined, 16 (18%) were found positive for Arcobacter. One Arcobacter species was isolated from each bird. Of the 16 Arcobacter isolates, 7 (44%), 7 (44%) and 2 (12.5%) were identified by m-PCR as A. cryaerophilus, A. skirrowii and A. butzleri, respectively. The present study indicates that domestic geese can harbour a variety of Arcobacter spp. in their cloacae. The presence of Arcobacter in geese may be of significance as reservoirs in their dissemination. Detailed research is needed for better understanding of the epidemiology and zoonotic potential of this emerging pathogen. #
The gum exudates leaking from cherry and apricot trees were used as edible coating material in this study. Some chemical properties of these gums were analysed whether they are proper as edible coating or not. Carbon, hydrogen, nitrogen and sulfur percentages were determined by elemental analysis. Both two gums were found rich in phenolic content and their antioxidant capacities were detected high as a result of the analysis. The behaviours against the heat and losses of weight of the films extracted from gums were determined by thermal analysis. According to these analysis, films were found heat tolerant up to 400°C. It was observed that the surface images of the films taken by SEM were homogeneous and a regular structure. Afterwards, strawberry and loquat fruits were coated with film solutions extracted from gums. The coated/uncoated fruits stored in refrigerator at 4±1°C were compared in respect of shelf life, organoleptic and microbiologic properties. It was seen that the films extended shelf life of the coated fruits. The total bacteria, yeast-mould and coliform bacteria levels of the uncoated samples were highest level at the end of the storage. Also, coatings affected positively organoleptic properties of the fruits. According to the sensory analysis test, the coated fruits had higher points from the uncoated fruits. The cherry and apricot tree gums are suitable materials to use as edible coating.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.