Background: A beta hemolyticus infection can cause a number of infections by different kinds of pathogenic mechanisms in the general population. These can range from streptococcal skin infections to rheumatic valve and heart diseases, as well as brain damage like encephalitis and paediatric autoimmune neuropsychiatric disorders. Objectives: The objective of this study was to find out the presence of anti-streptococcus antibodies in bipolar affective disorder with respect to family history, educational background, and gender differences. Methodology: The sample was collected with patient consent during the past year from the Bolan Medical College Complex Hospital, the Quetta Department of Psychiatry’s out door and in-door patients and from private referrals. A cross-sectional study with a sample size of 100 patients with a history of elated mood and low-grade fever in the absence of obvious causes, or if any causes were discovered after treatment, was conducted after carrying out their general physical and systemic examinations in order to include them in this study. Results: The positive anti streptolysin-o (ASO-titer) meaning more than 200 IU, is set for a positive result for the presence of the streptococcal antibody levels. In this study, the authors found a 29% prevalence of positivity. The variables are positive psychiatric family history, no education or poor educational background, and the third variable is the way the gender-based differences are, in males, the results are 71.7% and in females, it is 70% negative. Conclusion: The female gender, poor educational background, and positive family history are the main outcomes of this study.