SUMMARYThe entire plant of Leptadenia reticulata (Asclepidaceae) is extensively used as lactogen, traditionally, in veterinary practice. The plants of Dregea volubilis and Pentatropis microphylla (Asclepidaceae) are now used as its substitute and sometimes replace the original drug as traditional lactogen. The lactogenic potential of these drugs was studied in rats using, pup weight, weight of mother, parenchyma percentage, secretary rating, estimation of total protein content and glycogen content of mammary glands tissues as assessment parameters. HPTLC profiles of bioactive extracts were also generated to serve the authentification needs. The results of present studies show that P.microphylla forms a better substitute over D. volubilis.