To utilize timber and forest biomass resources for bioenergy, technically feasible and economically viable timber and forest biomass resources should be estimated accurately considering their long-term availability. This study focuses on five prefectures, namely, Aomori, Iwate, Miyagi, Akita, and Yamagata, and considers trade between these prefectures. The annual availability of timber and forest biomass resources, such as small-diameter or defect stem logs, rather than logging residues in Japan was estimated as supply potential from profitable forests where expected revenues surpassed all costs, from planting to final harvest. As a result, the supply potential and annual availability of timber were estimated at 11,388,960 m3/year and 1,631,624 m3/year, whereas those of forest biomass resources were estimated at 2,277,792 m3/year and 326,325 m3/year, respectively. Therefore, the rate of annual availability to supply potential was 14.3%. Since timber production and wood chip usage from thinned woods and logging residues in 2018 were 4,667,000 m3/year and 889,600 m3/year, the rates of annual availability for timber and forest biomass resources to those values were 35.0% and 36.7%, respectively. Furthermore, the demand was estimated at 951,740 m3/year from 100,000 m3/year with a generation capacity of 5 MW. The rate of forest biomass resource availability to demand was 34.2%. A thinning subsidy was provided for operational site areas larger than 5 ha in Japan. The rates from subcompartments and aggregated forests with a thinning subsidy increased to 91.4% and 190.3%, respectively. Thus, the estimated availability from aggregated forests with a thinning subsidy met the demand sufficiently in this region.