A kinetic method of the determination of kojic acid with 2,6-dichlorophenolindophenol(DCIP) is described. The oxidized form of DCIP, reacting with kojic acid, changes to the colorless reduced form of DCIP. By spectrophotometrically measuring the rate of discoloration, the apparent first-order rate constant, kapp, and the second-order rate constant, k, were obtained. The determination of kojic acid in the fermentation medium is possible by using k the constant, even in the presence of various organic substances.

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Six kinds of reductone were fractionated by high-performance liquid ckromatography (HPLC) using a SAX-801 Yanaco column and eluting with 0.1M NaNO3. Detection was accomplished by absorption at 260nm. By this method, 3-hydroxy-2-pyrone (HP) and 3, 4-dihydroxy-5-methyltetron (MDT) were separated from the solution of degradation products of L-ascorbic acid (ASA). The time required for HPLC analysis was approximately 30min, with detection limits of ca. 5.0×10-4 M for HP and 1.0×10-4M for MDT.