Cut Eustoma grandiflorum (Raf.) Shinn. flowers are produced year-round in Japan; however, winter conditions are not favorable for flower production due to low sunlight levels. Here, we investigated the effect of CO 2 enrichment after the flower budding stage on the growth and flowering of Eustoma 'Bolero White', which were grown under forcing culture for winter shipping. CO 2 enrichment increased the fresh weight of plants, in addition to increasing the dry weights of leaves, stems, flower buds and open flowers, and roots. CO 2 enrichment also increased the relative growth rate (RGR) by 32%, due to the net assimilation rate (NAR) being stimulated. However, CO 2 enrichment had no effect on plant height or the leaf area ratio (LAR).

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