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Since hydrogen is a renewable energy source, biohydrogen has been researched in recent years. However, data on hydrogen fermentation by a leachate from a waste landfill as inoculum are scarce. We investigated hydrogen production using a leachate from an industrial waste landfill in Kanagawa Prefecture. The results showed no methane gas production, and the leachate was a suitable inoculum for hydrogen fermentation. The maximum H 2 yield was 2.67 mol of H 2 per mol of carbohydrate added, obtained at 30°C and an initial pH of 7. The acetate and butyrate production was significant when the H 2 yield was higher. Oxidation-reduction potential analysis of the culture suggested that hydrogen-producing bacteria in the leachate were facultative anaerobic. Scanning electron microscope observations revealed that the hydrogen-producing bacteria comprised bacilli about 2 lm in length.

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