The current study included the detection of Toxoplasma gondii from aborted women from Maternity and Childhood Teaching Hospital. It drew attention to the human infection rate in order to detect the differentiation them with SAG3 gene. The samples were collected during the period from October, 2021 until the end of March 2022 in AL-Qadisyah province. Total of 100 samples (placenta and fetus) were collected and send to the laboratory of veterinary medicine AL- Qadisiyah University in order to detect women with toxoplasmosis by impression method all of are positive. The present study included a molecular method to detect surface antigen 3 (SAG3) using Nested Polymerase Chain Reaction (nPCR), the results of the nPCR for SAG3 gene showed that 18/24 (75 %) were positive in the aborted woman. the NCBI-BLAST Homology Sequence identity (%) between local T. gondii isolated from women cases that have been deposited in the NCBI under the following accession numbers (ON240954, ON240955, ON240956, ON240957, ON240958, ON240959, ON240960, ON240961, ON240962, and ON240963) and NCBI-BLAST deposited other global strains.

The study aimed to investigate the differences in the genetic tree of types infected (Free living and Industrial chickens) with the Toxoplasma parasite, (brain and liver) collecting samples in Al-Qadisiyah province during the period from October 2021 until the end of March 2022. A total of 90 samples of chicken were collected, 60 samples of brain and 30 samples of liver. The purpose of diagnosis, the impression method was used. It was found that 60 samples of free living chickens were all positive for examination. As for industrial chickens, 20 samples from 30 were positive at rate 88.8%, in rate of (83.3%) 50/60 from the brain and (66.6%) 20/30 from the liver.  Nested PCR targeting aspecific region within SAG3 gene technique applied for detect the genotype of T.gondii, the results showed that 30/48 of the chickens (brain and liver) (62.5%) were positive. A genetic match was found between Toxoplasma gondii in chickens SAG3 gene and the gene isolates according to NCBI – BLAST.