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The book entitled “Practical Manual of Biochemistry” is based on my experiences of hands on practical’s and teaching biochemistry, biotechnology and microbiology students of Undergraduate and postgraduate courses. This book has been written in a clear, logical manner to cover practical aspects of biochemistry. The book intended for undergraduate and post graduate students of biochemistry, chemistry, botany, zoology, biotechnology and microbiology. This manual contains experiments, which can be performed in any laboratory with basic requirements of chemicals and instruments. I shall be grateful for bringing out the mistakes and misprints, which shall be removed in the next generation. Comments and suggestions from readers for corrections and improvement of this book will be greatly acknowledged. I am thankful to my students, who encourages me to publish my regular notes in manual format. I am also thankful to publisher for taking keen interest and bringing out the book in present form. Finally I am grateful to my family and relatives for inspiring me in proceeding from thought to print.

The book is designed to provide a comprehensive coverage in the area of organic reaction mechanism for a chemistry major/graduate student. The practice of medicine now-a-days increasingly demands a knowledge of the behaviour of molecules. Therefore, the biologist of tomorrow will have to be more of an organic chemist, among other things. The book is thus aimed at Pharmacologists, Medical chemists and Biochemists.

In the present work, Zn(II), Co(II) and Cr(II) metal complexes (3-5) of N-hydroxy-4-[(hydroxyimino)methyl]benzamidine ligand (2) were synthesized and their anti-HIV activity by inhibiting reverse transcriptase was explored. Structural characterization using NMR, FTIR, Mass, UV-Vis spectra, TGA, magnetic moment and elemental analyses were performed. The analyses show that geometry, lipophilicity and steric factor play crucial role. Cell viability test is performed on peripheral blood mononuclear cells to check toxicity of prepared compounds on immune system, shows toxicity only at higher concentration. Antimicrobial activity and DNA cleavage analysis have also been studied and reported.