The genus Blumea, belonging to the family Asteraceae, comprises 80 species of small annual weeds. The plants of this genus are widely distributed in tropical and subtropical Asia, Africa, and Oceania. The plants of this genus are mostly small annual weeds and are of great medicinal value. Some of these species are used as folk medicines for treating colds, fevers, blood diseases, dysentery, gynecological diseases. 1,2 These have provided a variety of constituents, including flavonoids, monoterpenes, sesquiterpenes, acetylenic thiophenes, triterpenoids, xanthenes, diterpenes, and essential oils. 1 Blumea lacera (Burn. f.) DC, a herbaceous weed named "Cai troi" in Vietnam, is mainly distributed in Lao Cai, Vinh Phuc and Ha Giang provinces in Vietnam. 3 It is also commonly found in China, India, Bangladesh, Australia and tropical Africa. 4 The plant has long been used in traditional medicine as expectorant, diuretic, astringent, antispasmodic, antipyretic, antioxidant, antidiarrheal, liver tonic and stimulant. 5,6 Previous biological studies have shown that extracts of B. lacera exhibit antiviral, 7 anti-leukemic 7 , antiulcer 6 and cytotoxic activities against several human cancer cell lines. 8,9 In addition, it has been reported that essential oil from this herb exhibit analgesic, hypothermic, and tranquilizing activities and cytotoxic activities against breast cancer cells and healing cuts. 6,10 Several investigations into the secondary metabolites of B. lacera have revealed the presence of flavonoids, terpene glycosides, phenol glycosides, sterols, essential oils, coniferyl alcohol derivatives, terpenoid ketones and steroidal glycoalkaloids. 4,[8][9][10][11][12][13][14] To increase the value of this herb in terms of its potential application for medicinal purposes, it was considered necessary to investigate its chemical constituents and to understand their biological properties. MATERIALS AND METHODS General experimental procedureThe NMR spectra were measured using a Varian Unity-Inova 400 MHz spectrometer. The solvents used for extraction and isolation were of analytical grade solvents. Silica gel (63-200 mm; Merck, Darmstadt, Germany) and RP-18 (75 mm; Merck) were used for column chromatography. Thin layer chromatography was carried out on pre-coated silica gel 60 F254 plates and RP-18 F254 plates (both from Merck) and the plates were visualized by spraying with 10% H 2 SO 4 /EtOH solution followed by warming.