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Epidemiological surveys in Algeria conducted in the school health program show that the dental carious prevalence is about 67%. Water is the main source of fluorine, recognized as the essential chemical element to prevent carious dental For this purpose, the objective of this study is to test, in vitro, the inhibitory effect of Saharan fluoridated water for the dissolution of hydroxyapatite Ca5(PO4)3OH, under physiological conditions: acid (HNO3) at pH = 5.1 and T = 37 ° C. Thence, the fluoride content of drinking water of some Saharan region was measured, it varies from 0.48 to 3.87 ± 0.05 mg / L.The Inhibitory effect of waters is between 54.28 and 83.1%, VC <5%. In addition, a low concentration of fluorine [F-] = 0.25 mg / L reduced the solubility (S) of hydroxyapatite by a factor of 2. Thus, the studied Sahara waters inhibit ‘in vitro’ the dissolution of hydroxyapatite Ca5 (PO4)3OH.