Background: Acute myeloid leukemia (AML) is a hematopoietic neoplasm. Tumor suppressors have a magnificent role in preventing the AML process. The absolute lymphocyte count is a simple yet statistically powerful estimate in patients with acute leukemia besides the lymphocyte’s percentage. Aim: Investigating the prognostic value of absolute lymphocyte count, lymphocyte percentage, serum albumin, the aberrant expression of CD7and CD19 and the tumor suppressor genes (PTEN and p53) in patients with AML. Methods: 35 de novo AML patients were included. They received the standard induction chemotherapy (3+7 protocol) and were followed up for one year after treatment. 15 normal healthy individuals, age and sex matched constituted the controls.Results: The mean overall survival of patients with lymphocyte percentage ≤25 was low compared to those with high lymphocyte percentage (>25%) (χ2 =5.808, P=0.016). AML patients with low levels of ALC showed significantly shorter overall survival than patients with high levels (χ2 =4.587, P= 0.032). AML patients with low serum albumin were of low overall survival compared to those with normal level (χ2 =8.698, P=0.003). Patients with aberrant CD7 expression showed short survival and unresponsiveness to treatment than CD7 negative patients. PTEN gene expression and p53 protein level were significantly lower in AML patients compared to the control group.Conclusion: The decrease in ALC, lymphocyte percentage, albumin concentration and the increase in monocyte percentage indicates bad prognosis in AML patients. The Aberrant CD7 expression, very low expression of PTEN and low level of p53 could estimate the unresponsiveness to standard chemotherapy.

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Background: Toxoplasma gondii is a highly frequent obligate intracellular protozoan parasite; it can cause serious problems to the public health especially pregnant females, however, the pathogenesis of this condition is not clear. Objective: was to evaluate the status and the interrelationship of the oxidative stress and platelet activation in patients infected with T. gondii. Methods: Thirty patients infected with T.gondii (10 acute and 20 chronic cases) and 10 healthy subjects (control group) were included in this study. Serum levels of malondialdehyde (MDA), total glutathione (t GSH), reduced glutathione (GSH), oxidized glutathione (GSSG), redox potential (RP) and soluble P-selectin (sPselectin) were measured. EDITA blood samples were used for complete blood picture with special emphasis on platelet count and mean platelet volume (MPV). Results: The mean platelet volume (MPV) in patients was significantly higher than the control group, however, platelet count showed no significant difference. The serum mean values of MDA, GSSG, RP and sP-selectin in patients were significantly higher than the control subjects. On the other hand, the levels of tGSH and GSHin patients were significantly lower than in controls. MPV and platelet count showed significant positive correlations with sP-selectin concentration. Conclusion: Although toxoplasmosis is mostly asymptomatic, the findings of the present study strongly indicate that the occurrence of oxidative stress could be a potential mechanism of subclinical inflammatory pathology and tissue damage in these cases.