Vitamin D is known to elicit a vasoprotective effect, while vitamin D deficiency is a risk factor for endothelial dysfunction (ED). ED is characterized by reduced bioavailability of a potent endothelium-dependent vasodilator, nitric oxide (NO), and is an early event in the development of atherosclerosis. In endothelial cells, vitamin D regulates NO synthesis by mediating the activity of the endothelial NO synthase (eNOS). Under pathogenic conditions, the oxidative stress caused by excessive production of reactive oxygen species (ROS) facilitates NO degradation and suppresses NO synthesis, consequently reducing NO bioavailability. Vitamin D, however, counteracts the activity of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase which produces ROS, and improves antioxidant capacity by enhancing the activity of antioxidative enzymes such as superoxide dismutase. In addition to ROS, proinflammatory mediators such as TNF-α and IL-6 are risk factors for ED, restraining NO and eNOS bioactivity and upregulating the expression of various atherosclerotic factors through the NF-κB pathway. These proinflammatory activities are inhibited by vitamin D by suppressing NF-κB signaling and production of proinflammatory cytokines. In this review, we discuss the diverse activities of vitamin D in regulating NO bioavailability and endothelial function.

Kisspeptin has recently emerged as a key regulator of the mammalian reproductive axis. It is known that kisspeptin, acting centrally via the kisspeptin receptor, stimulates secretion of gonadotrophin releasing hormone (GnRH). Loss of kisspeptin signaling causes hypogonadotrophic hypogonadism in humans and other mammals. Kisspeptin interacts with other neuropeptides such as neurokinin B and dynorphin, to regulate GnRH pulse generation. In addition, a growing body of evidence suggests that kisspeptin signaling be regulated by nutritional status and stress. Kisspeptin may also represent a novel potential therapeutic target in the treatment of fertility disorders. Early human studies suggest that peripheral exogenous kisspeptin administration stimulates gonadotrophin release in healthy adults and in patients with certain forms of infertility. This review aims to concisely summarize what is known about kisspeptin as a regulator of reproductive function, and provide an update on recent advances within this field.

Resting metabolic rate (RMR) and substrate oxidation (respiratory exchange ratio; RER) are important indicators of health. The effects of interval training on RMR have not been thoroughly investigated, which was the purpose of the present study. Thirty men and women (mean ± SD age and maximal oxygen uptake: 28.8 ± 7.6 years and 33.0 ± 8.3 mL·kg·min) completed 4 weeks of Wingate-based sprint interval training (SIT), repeated 1-min high-intensity intervals (HIIT), or served as controls. Before and after training, RMR, resting RER, maximal oxygen uptake, body composition, physical activity, and energy intake were recorded. Data were analyzed using a repeated-measures ANOVA. RMR increased in response to 4 weeks of SIT training (1789 ± 293 to 1855 ± 320 kcal·day; p = 0.003) but did not increase after HIIT (1670 ± 324 to 1704 ± 329 kcal·day; p = 0.06). While SIT increased RMR by ∼2× the magnitude of HIIT, the difference was not significant (p = 0.5). Fasting substrate oxidation and RER did not change (p > 0.05). Maximal oxygen uptake increased, and small changes were also observed in percent body fat and fat mass (p < 0.05 for all). In conclusion, SIT provided a time-efficient stimulus to increase RMR after 4 weeks in healthy adults. However, the clinical relevance of the changes observed in this study remains to be determined. Further studies should be conducted in obese individuals and those with diabetes or insulin resistance to examine if interval training (≥4 weeks) influences resting metabolic rate in magnitudes similar to that reported here.

Summary Accurate measures of body composition (BC) are essential for performance and health. In addition to accuracy, BC measures should be practical and be minimally invasive to maximize their utility. The purpose of the present study was to compare the day‐to‐day variability and validity of four common laboratory‐based body composition assessments to a criterion four‐compartment model. Dual x‐ray absorptiometry (DXA), air displacement plethysmography (BP), multi‐frequency bioelectrical impedance (MF‐BIA) and underwater weighing (UWW) were performed twice in a sample of 32 young men and women. Participants were assessed in a fasted, euhydrated state 2–7 days apart. All methods were compared to a criterion four‐compartment model using BP‐derived body volume, DXA‐derived bone mineral content and MF‐BIA‐derived total body water (4CBP). Additional four‐compartment models using UWW‐ and DXA‐derived body volume were also examined (4CUWW) and (4CDXA). Validity results were conducted with paired t‐tests and Bland–Altman analysis. Reliability was determined using intraclass correlations (ICC), coefficients of variation (CV) and standard error of the measurement (SEM). Validity analysis revealed that all methods overestimated per cent body fat and fat mass, and underestimated fat‐free mass when compared with 4CBP, but only DXA and BP were significantly different (P<0·008). All measures were highly reliable across days (ICCs > 0·9, CVs < 12%). Results of the present study indicate that typical laboratory‐based methods of body composition are valid and reliable. However, we caution that results should not be translated between methods and assessments should be performed with the same instrument when the goal is to monitor changes in body composition over time.