Purpose: “Yan Shou Hong Cui” is a representative punch in Chen-style Tai Chi. The training is an important factor in affecting the effect of Tai Chi practice. Joint angles are the most intuitive way to evaluate motion. The purpose of this study is to compare the movements of Tai Chi masters and beginners’ movements through the analysis of joint angle and punching effect, and explore the influence of training years on the power generation of Tai Chi punches. Methods: There were 14 Chinese Chen-style Tai Chi subjects recruited for this study. They were divided into the master group (n = 7, age of 38.14 ± 10.42 years, height of 1.68 ± 0.06 m, weight of 71.33 ± 8.61 kg) and the beginner group (n = 7, age of 38.00 ± 11.94 years, height of 1.69 ± 0.07 m, weight of 70.14 ± 9.79 kg). The typical movement from Chen-style Tai Chi is called Yan Shou Hong Cui. All subjects were asked to perform the action three times, and the way of force was applied each time. The kinematic and kinetic characteristics of Tai Chi were analyzed by the VICON 3D motion analysis system (100 Hz) with 16 MX13 cameras, and the punch efficiency was measured by the Chinese Kung Fu Dummy (80,000 Hz). Results: The experimental results show that the shoulder, elbow, and hand movements of the master group are more precise and the force strength is more vigorous (master group: the peak angular velocity of the shoulder joint was −324.12 ± 50.88°/s, the angular velocity of the elbow joint was −112.83 ± 56.98°/s, and the hand angular velocity was −121.69 ± 49.55°/s; beginner group: shoulder angle velocity was −281.17 ± 30.56°/s, the elbow angle speed was −263.64 ± 68.63°/s, and the hand angle speed was −36.80 ± 12.53°/s). The rotation angle and rotation speed of the hip joint in the master group were significantly higher than those in the beginner group (as for the hip rotation angle, the master group was 64°, which was significantly higher than the beginner group’s 44°, and there was a significant difference (p = 0.019 < 0.05)). In terms of rotation speed, the peak value of the master group was 370.3 ± 94.8°/s, which was significantly faster than that of the beginner group at 210.4 ± 56.1°/s, and there was a very significant difference (p = 0.003 < 0.01). The master group’s punch effect acceleration (496.39 ± 256.52 m/s2) was significantly higher than that of the beginner group (396.90 ± 116.79 m/s2). Conclusion: People who practice Tai Chi for a long time differ from the beginners in terms of joint flexibility, punching posture, and the effect of application.