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Objective: The objective of this study was aimed to investigate the interactions between Aurora kinase A (AURKA) gene polymorphisms (T91A and G169A) and smoking and their effects on the susceptibility of oral cancer. Methods: One hundred five healthy controls were frequency-matched with 91 oral cancer patients by age, sex and nationality. Detection of AURKA T91A and G169A polymorphisms was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Differences in genotypes and alleles between case and control groups were compared using v 2 test. Relative risk of oral cancer was presented as odds ratios (ORs) and 95% confidence intervals (95% CIs). Results: AA genotypes respectively of AURKA T91A and G169A polymorphisms had higher frequencies in case group than in control group (p ¼ .023; p ¼ .038). However, only 91AA genotype was significantly associated with the occurrence of oral cancer risk (OR ¼ 3.113, 95% CI ¼ 1.176-8.236). A alleles of the two polymorphisms respectively increased the risk of oral cancer (OR ¼ 1.700, 95% CI ¼ 1.113-2.596; OR ¼ 1.978, 95% CI ¼ 1.132-3.454). Interactions between smoking and AURKA polymorphisms significantly associated with the onset of oral cancer (p < .05). Conclusions: AURKA polymorphisms are susceptible factors for oral cancer. Interactions between AURKA polymorphisms and smoking increase the risk of oral cancer.

Previous studies have shown that microRNA‐206 (miR‐206) exhibits anti‐tumour properties in various tumours. Nevertheless, diagnostic significance of miR‐206 in oral cancer is still poorly known. Our research was carried out to explore the performance of miR‐206 in the diagnosis of oral cancer. Quantitative real‐time polymerase chain reaction (qRT‐PCR) method was adopted to measure the level of miR‐206 in serum specimens from oral cancer cases and control individuals. Chi‐square test was performed to analyse the correlation between miR‐206 level and clinicopathological parameters of the cases. Receiver operating characteristic (ROC) curve was constituted to assess diagnostic accuracy of miR‐206 in oral cancer. Serum miR‐206 level in oral cancer patients was significantly lower than that in control individuals (P < .001). miR‐206 expression was obviously related to T classification (P = .033), TNM stage (P = .008) and lymph node metastasis (P = .028). The area under the curve (AUC) of the ROC curve was 0.846 (95% CI = 0.797‐0.896, P < .001) with a specificity of 72.7% and a sensitivity of 81.2%. It revealed that miR‐206 might be a non‐invasive indicator in differentiating oral cancer cases from control individuals. Down‐regulation of miR‐206 is related to the development of oral cancer. Serum miR‐206 might be an effective indicator for early detection of oral cancer.

Background: The aim of this study was to assess the prognostic value of Krüppel-like factor 7 (KLF7) for patients with oral squamous cell carcinoma(OSCC).Methods: The expression of KLF7 was detected by quantitative real-time polymerase chain reaction (qRT-PCR) in pairs of tumor tissues and adjacent non-tumor tissues of OSCC. Chi-square (χ2) test was applied to evaluate the association of KLF7 expression with clinicopathological characteristics of OSCC patients. Overall survival was estimated using the Kaplan-Meier method with log rank test. The cox proportional hazards model was used for univariate and multivariate analyses.Results: The expression of KLF7 was remarkably increased in OSCC tissues compared with adjacent non-tumor tissues (P<0.001). KLF7 expression was related to TNM stage (P=0.006), tumor size (P=0.010), smoking (P=0.006) and drinking (P=0.000). Kaplan-Meier analysis showed that OSCC patients with high KLF7 expression had a poorer overall survival than those with low expression (log rank test, P=0.018). Moreover, multivariate analyses showed that KLF7 was an independent prognostic factor for OSCC (P=0.002 HR=2.645 95%CI: 1.426-4.906).Conclusion: Decreased expression of KLF7 may be a potential unfavorable prognostic factor for patients with OSCC.

Background Oral cancer is a common malignant tumor in head and neck with poor prognosis. This study aimed to determine the expression tendency and prognostic value of PDGFRβ in oral cancer. Methods The mRNA expression level of PDGFRβ in the oral cancer tissues and adjacent normal tissues of oral cancer patients were detected by quantitative real-time polymerase chain reaction (qRT-PCR). And the association of PDGFRβ expression with clinicopathological characteristic was analyzed via chi-square test. Then we used Kaplan-Meier analysis to analyze the effects of PDGFRβ expression on the overall survival of oral cancer patients. The multivariate cox analysis was used to evaluate its prognostic value. Results The results indicated that the mRNA expression level of PDGFRβ was significantly increased in oral cancer tissues compared with that in the adjacent normal tissue ( P < 0.001). And its expression is positively associated with clinical stage, T stage, lymph node metastasis and histological grade. Kaplan-Meier analysis revealed that patients with high expression of PDGFRβ had markedly worse overall survival than those with low expression of PDGFRβ (log rank test, P < 0.05). Additionally, cox regression analysis revealed that the high expression of PDGFRβ was an independent prognostic maker in oral cancer patients. Conclusion PDGFRβ is up-regulated and involved in the development of oral cancer. Moreover, it could be an independent prognostic bio-marker for oral cancer.