Gardenoside is one of the most important effective extractions of a herb for its hepatoprotective properties. The aim of this study was to address the mechanism of Gardenoside on HepG2 cellular steatosis induced by free fatty acids (FFAs). The model of HepG2 steatosis was duplicated by oleic and palmitic acid at the proportion of 2:1 (FFAs mixture) for 24 h, then lipid toxicity was induced. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were used to detect cell viability and Oil Red O staining method was used to judge the lipid accumulation respectively. Inflammatory cytokines TNF-α, IL-1β, IL-6 and intracellular NFκB were measured after 24 h. The steatosis was significantly decreased after Gardenoside treatment without cytotoxicity. TNF-α, IL-1β, IL-6 were modulated to HepG2 cells by treatment of Gardenoside. In the meantime, the activation of NFκB was inhibited by Gardenoside. Gardenoside has a protective effect on FFA-induced cellular steatosis in HepG2 cells which indicates that Gardenoside might be a potential therapeutic herb against NASH by suppressed supernatant inflammatory cytokine production and intracellular NFkB activity.

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Snow water equivalent (SWE) is a critical parameter for characterizing snowpack, which has a direct influence on the hydrological cycle, especially over high terrain. In this study, SWE from 18 coupled model simulations from phase 5 of the Coupled Model Intercomparison Project (CMIP5) is validated against the Canadian Sea Ice and Snow Evolution Network (CanSISE) SWE. The model simulations under RCP8.5 and RCP4.5 are employed to investigate projected changes in spring/winter SWE over the Tibetan Plateau (TP) under global warming of 1.5° and 2°C. Most CMIP5 models overestimate the CanSISE SWE. A decrease in mean spring/winter SWE for both RCPs over most regions of the TP is predicted in the future, with most significant reductions over the western TP, consistent with pronounced warming in that region. This is supported by strong positive correlations between SWE and mean temperature in the future in both seasons. Compared with the preindustrial period, spring/winter SWE over the TP under global warming of 1.5° and 2°C will reduce significantly, at faster rates than over China as a whole and the Northern Hemisphere. SWE changes over the TP do not show a simple elevation dependency under global warming of 1.5° and 2°C, with maximum changes in the elevation band of 4000–4500 m. Moreover, there are also strong positive correlations between projected SWE and historical mean SWE, indicating that the initial conditions of SWE are an important parameter of future SWE under specific global warming scenarios.

Near-infrared fluorescence imaging plays an important role in the diagnosis and treatment of major diseases by virtue of its high sensitivity and specificity. However, compared with the traditional NIR-I (700∼900 nm) fluorescence imaging, NIR-II (900∼1400 nm) fluorescence imaging has the advantages of higher tissue penetration depth and higher imaging signal-to-noise ratio in vivo imaging studies. The review focuses on the development of NIR-II fluorescent probes in recent years, classifies and discusses the activable NIR-II fluorescent probes, and systematically introduces the microenvironment-responsive fluorescent probes. The research results of redox activated probes, pH activated probes, hypoxic activated probes, enzyme activated probes, viscosity activated probes, ATP and metal ion activated probes in the field of biological applications are included. Finally, the development prospect of NIR-II fluorescent probe is prospected.

Platinum-based chemotherapy is still the standard of care after cytoreductive surgery in the first-line treatment for epithelial ovarian cancer. This study aims to integrate novel biomarkers for predicting platinum sensitivity in EOC after initial cytoreductive surgery precisely. To this end, 60 patients were recruited from September 2014 to October 2019. Based on the duration of progress-free survival, 44 and 16 patients were assigned to platinum-sensitive and platinum-resistant group, respectively. Next generation sequencing was performed to dissect the genomic features of ovarian tumors obtained from surgery. Multiple genomic variations were compared between two groups, including single-nucleotide variant, single base or indel signature, loss of heterozygosity (LOH), whole-genome duplication (WGD), and others. The results demonstrated that patients with characteristics including positive SBS10a signature (p < 0.05), or FAM175A LOH (p < 0.01), or negative WGD (p < 0.01) were significantly enriched in platinum-sensitive group. Consistently, patients with positive SBS10a signature (15.8 vs. 10.1 months, p < 0.05), or FAM175A LOH (16.5 vs. 9.2 months, p < 0.05), or negative WGD (16.5 vs. 9.1 months, p < 0.05) have significantly longer PFS than those without these genetic features. By integrating these three biomarkers, a lasso regression model was employed to train and test for all patients, with the AUC value 0.864 in platinum sensitivity prediction. Notably, 388 ovarian cancer patients from TCGA dataset were leveraged as independent validation cohort with AUC value 0.808, suggesting the favorable performance and reliability of this model.