Lactic acid fermentation is a promising method for developing sandwich seaweed scraps. The objectives of this study were to investigate the effect of fermentation with Lactiplantibacillus plantarum MMB-05, Lactiplantibacillus casei FJAT-7928, mixed bacteria (1:1, v/v) and control on the physicochemical indexes, in vitro antioxidant activity, and volatile compounds of Porphyra yezoensis sauce. Sensory evaluation was also performed. The results indicated that all lactic acid bacteria strains grew well in P. yezoensis sauce after 72 h of fermentation, with the viable cell counts of L. plantarum MMB-05 exceeding 10.0 log CFU/mL, the total phenolic content increasing by 16.54%, and the lactic acid content increasing from 0 to 44.38 ± 0.11 mg/mL. Moreover, the metabolism of these strains significantly increased the content of umami, sweet and sour free amino acids in P. yezoensis sauce. The total antioxidant capacity of L. plantarum MMB-05, L. casei FJAT-7928, mix and control groups increased by 594.59%, 386.49%, 410.27%, and 287.62%, respectively. Gas chromatography-ion mobility spectrometry (GC-IMS) analysis suggested that aldehydes and ketones accounted for the largest proportion, and the relative contents of acids and alcohols in P. yezoensis sauce increased significantly after lactic acid bacteria fermentation. In addition, the analysis of dynamic principal component analysis (PCA) and fingerprinting showed that the volatile components of the four treatment methods could be significantly distinguished. Overall, the L. plantarum MMB-05 could be recommended as an appropriate starter for fermentation of sandwich seaweed scraps, which provides a fundamental knowledge for the utilization of sandwiched seaweed scraps.
To explore an effective quality evaluation system based on microstructure changes, tilapia samples were preserved at the temperature of −20, −40, and −80°C up to a storage period of 270 days. The porous structure of fish muscle was quantified using the cross‐section area (CA) and fractal dimension (FD) of ice crystals, and the effectiveness of the two was compared. The relationships between microstructure changes and traditional quality parameters, including water‐holding capacity, texture properties, K value, and total volatile basic nitrogen (TVB‐N), were also explored. The results showed that FD dropped by 8.79, 4.53, and 3.03% of fillets at −20, −40, and −80°C after 270 days of storage, respectively. The better fitting on the basis of FD for evaluating quality of tilapia compared with CA. Traditional quality indicators were correlated well with the FD (R2 > 0.94), except for relaxation time (T2). The first‐order kinetic equation FD = exp (0.0895 t exp[−7240.26/RT])/1.8903 was developed, and FD relative errors between predicted and observed values were all less than 3%, which demonstrated that the feasibleness and accuracy of quality evaluation based on FD during frozen storage. Practical applications Frozen storage inevitably has a negative impact on the tissue, water‐holding capacity, and protein properties of fish. Thus, it is of great urgency to put forward an effective quality evaluation system of frozen fish. Here, tilapia samples were studied over a period of 270 days by measuring their microstructure of muscle fibers and traditional quality parameters. Correlation analysis of each index was also conducted and kinetic model based on FD was established and validated finally. In addition to establishing an effective quality evaluation system based on microstructure changes for tilapia, these results will provide new insights into the quality evaluation and laid a foundation for the application of fractal dimension in tilapia processing and transportation.
To provide recommendations to users regarding which thawing method for frozen instant sea cucumbers entails lower quality losses, in this study we compared the water retention, mechanical properties, protein properties, and microstructures of frozen instant sea cucumbers post-thawing by means of different thawing approaches, including refrigerator thawing (RT), air thawing (AT), water immersion thawing (WT), and ultrasound-assisted thawing (UT). The results indicated that UT took the shortest time. RT samples exhibited the best water-holding capacity, hardness and rheological properties, followed by UT samples. The α-helix and surface hydrophobicity of WT and AT samples were significantly lower than those of the first two methods (p < 0.05). The lowest protein maximum denaturation temperature (Tmax) was obtained by means of WT. AT samples had the lowest maximum fluorescence emission wavelength (λmax). Based on these results, WT and AT were more prone to the degradation of protein thermal stability and the destruction of the protein structure. Similarly, more crimping and fractures of the samples after WT and AT were observed in the sea cucumbers’ microstructures. Overall, we observed that UT can be used to maintain the quality of frozen instant sea cucumbers in the shortest time.
The cover image is based on the Full Article Study on changes in the quality of grass carp in the process of postmortem, by Hongli Wang et al., DOI .
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