is study developed an efficient method to simultaneously separate and purify syringoside and oleuropein from Syringa oblata Lindl. extract using macroporous resins. e adsorption and desorption property of 11 resins were systematically evaluated. Based on the adsorption performance, HPD-100B resin was selected for the separation of syringoside and oleuropein. e HPD-100B resin fitted well to the Langmuir isotherm model (R 2 > 0.97), as ascertained by the results of the static adsorption experiment. Kinetic and dynamic adsorption/desorption experiments were conducted using the HPD-100B resin to optimize the separation parameters of syringoside and oleuropein. On the optimal parameters, syringoside and oleuropein were obtained from the 20% and 40% ethanol eluates, respectively. In addition, the adsorption effluent (15-60 BV) contained a large amount of syringoside with less impurities; therefore, this part was also collected for further syringoside separation and enrichment of syringoside. By only one cycle treatment, the syringoside and oleuropein contents in the final products increased by 7.1-fold and 8.2-fold, respectively, compared to the initial extract. e method developed in this study provides a potential basis for the industrial-scale enrichment and separation of syringoside and oleuropein from S. oblata extract.