Background and Objective: Bacterial and fungal infections are major public health problems. Emerging of drug-resistant microbial strains urges the need for the development of alternative untraditional antimicrobial agents. Bee venom is a rich source of secondary metabolites and antimicrobial agents. In this study, the antimicrobial and antifungal potential of Apis mellifera BV (AmBV) against some medically important bacterial and fungal pathogens was investigated. Materials and Methods: Broth microdilution method and Colony Forming Unit (CFU) assay were used to screen the antibacterial potential of AmBV. Similarly, the antifungal activity of AmBV was evaluated using the agar-well diffusion assay. Moreover, the minimum inhibitory concentration (MIC) values of AmBV against tested microorganisms were determined. Results: AmBV significantly inhibited bacterial and fungal growth.