During recent years, much attention has been paid to the analysis of fire effect on steel structures because of fire importance and its effect on the stability of steel structures. Considering steel sensitivity to heat and high costs of steel frames retrofitting with the help of thermal covers, accurate behavior analysis of metal frames in elevated temperatures is required in order to reduce undesirable effects of temperature increase. To do so and taking into account the high costs of lab tests and their limitations in investigation of numerous parameters during any test, finite element method is used as a powerful and valuable tool in modeling of steel frames under thermal conditions. In this study, the fire effect on steel beams is studied considering the softening of connection and decrease of materials strength. Then, structure durability in fire will be analyzed in the ANSYS software. The analyzed frame is a single span three dimensional one and different conditions including connection type, longitudinal expansion effect, thermal loading and the kind of fire will be analyzed and compared. The obtained computer results will be compared with other researches results.