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Chondroitinase ABCI (cABCI) is a drug enzyme that can be used to treat spinal cord injuries. Due to low thermal stability of cABCI, this enzyme was immobilized on Fe 3 O 4 nanoparticle to increase its thermal stability. The size and morphology, structure and magnetic property of the Fe 3 O 4 nanoparticles were characterized by the analyses of SEM, XRD and VSM, respectively, and FTIR spectroscopy was employed to confirm the immobilization of cABCI on the surface of Fe 3 O 4 nanoparticles. The results indicated that the optimum conditions for pH, temperature, cABCI-to-Fe 3 O 4 mass ratio and incubation time in immobilization process were 6.5, 15 C, 0.75 and 4.5 h, respectively, and about 0.037 mg cABCI was bound to 1 mg of Fe 3 O 4 nanoparticles at these conditions. The value of V max was the same for free and immobilized cABCI, but K m value for immobilized cABCI was 1.6 times higher than that for free one. The storage stability of immobilized cABCI was significantly enhanced at low temperatures, e.g. free cABCI retained 19% of its activity after six days at-20 C, while the immobilized one retained 96% of its activity. In vitro release of cABCI from Fe 3 O 4 particles showed that about 94% of the enzyme was released after 6 h.

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