Silver nanoparticles (AgNPs) have been used widely in medical applications and various industries. Humans could be exposed to the risk of AgNPs toxicity through different routes. The current study aimed to investigate the role of Spirulina platensis (SP) against the side effects of AgNPs on mice testis. Adult male NMRI mice were divided into four groups: control group, SP group (300 mg/kg bwt), AgNPs (20 nm) group (500 mg/kg bwt), Co-treated group (SP + AgNPs). The groups were treated orally for 35 days. Subsequently, epididymal sperm parameters, sperm DNA integrity, daily sperm production (DSP), sexual hormones level, malondialdehyde (MDA), total antioxidant capacity (TAC) and spermatogenesis indices were measured. In addition, the histopathology of testes was evaluated using tissue processing, haematoxylineosin staining and stereology techniques. A significant decrease in the number of spermatogenic cells, Leydig cells and sperm parameters was observed in the AgNPs treated group. Serum levels of testosterone and TAC were decreased significantly following AgNPs treatment. Also, MDA incremented in the serum of AgNPs treated mice. The stereological analysis revealed that AgNPs exposure induced histopathological changes in the seminiferous tubules, degeneration and dissociation of spermatogenic cells. In contrast, SP co-administration significantly counteracted AgNPs reproductive toxicity impacts. SP co-exposure caused an increase in spermatogenesis indices, TAC and also a decrease in MDA. SP improved the histopathological changes of testes tissue and spermatozoa abnormalities. In parallel, SP modulated levels of testosterone, FSH and LH. Spirulina platensis exhibited the protective potential by regulating oxidative stress against AgNPs-induced reproductive toxicity. SP could be a candidate therapy against AgNPs reprotoxic impacts.