Successful cultivation is difficult to pomegranate growers due to Xanthomonas axonopodis pv. punicae which cause bacterial blight disease to pomegranate has become serious threat. However, everyone is looking for solution; this problem is resolved by synthesizing silver nanoparticles by the reduction of silver ions in aqueous solutions with the help of green synthesis method. The causative agent was isolated from the infected pomegranate fruits and was tested with silver nanoparticles which are synthesized from leaf extracts of tulsi and neem; and it was found that silver nanoparticle from tulsi extract with 15 mM concentration showed maximum zone of inhibition 22 mm and 19 mm for neem, while at 1 mM concentration shows minimum. This study showed that synthesized silver nanoparticles are powerful weapon to control the growth of Xanthomonas axonopodis pv. punicae.

In this study, simple approach was applied for the synthesis, characterization and application of silver nanoparticles from the leaves of Bryophyllum p., garlic and jamun which is cost-effective, eco-friendly and easy to synthesis. The extract acts both as reducing as well as capping agent. The color change of the solution yellowish brown to reddish brown confirms the formation of silver nanoparticles. The synthesized silver nanoparticles were characterized by UV-visible spectroscopy which showed a peak between 420 and 430 nm corresponding to the absorbance of the AgNPs that confirmed the reduction of Ag + to Ag 0 nanoparticles. The anti-bacterial activity of AgNPs was investigated against Xanthomonas axonopodis pv. punicae and its identity was confirmed by morphological, biochemical and pathogenicity test. The results indicated that garlic AgNPs shoed maximum inhibition at a concentration of 15 mM. Hence, they can be used in the disease management in pomegranate.