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Eighteen animals were engaged for therapeutic trial with three groups (Group 1; Control, Group 2; Mixture of Ammonium Chloride and Calcium Sulphate at 1:1 ratio and Group 3; Commercial salts preparation, Hyporid™) containing six animals each. The efficacy of various anionic salt preparations was determined on the basis of haemato-biochemical changes and incidence of milk fever after calving. Dietary Cation-Anion Difference (DCAD) concentration in control group was positive (+25.22 mEq/100gm of dry matter). After adding anionic salt preparation DACD concentrations were negative in both Group 2 (-11.44 mEq/100gm of dry matter) and Group 3 (-8.26 mEq/100gm of dry matter). In this therapeutic study, milk fever incidence rate was 33.33% (2/6) in control group whereas no occurrence of milk fever was reported in Group 2 and Group 3. The mean value of calcium, phosphorus, chloride and sulphate was significantly lower (p<0.05) with significant increase (p<0.05) in mean value of magnesium and sodium in Group 1 compared to Group 2 and Group 3 after calving.