Although the gene clusters encoding Tfp are commonly present in Streptococcus sanguinis , not all strains express surface-dependent twitching motility on agar surfaces. Regardless of whether the Tfp could drive motility, Tfp can serve as a ligand for the colonization of host cells.

Organic agricultural tourism is an environment-friendly tourism that has emerged in recent years. However, no comprehensive dependency theory discusses the tourists’ pro-environmental behavior and well-being from the perspective of the public sphere. This research aims to verify the effect of the four dimensions of attachment and its impact on pro-environmental behavior. This research substantiated that the four aspects of attachment theory had a positive and significant influence on pro-environmental behavior and well-being. Furthermore, place and activity attachment had the highest impact. The results serve as a basis for understanding and motivating tourists’ pro-environmental behaviors and assist them in achieving well-being through organic agriculture tourism. This research also suggests sustainable development practices for destination operators or managers.

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CodY is a dominant regulator in low G + C, Gram-positive Firmicutes that governs the regulation of various metabolic pathways and cellular processes. By using various bioinformatics analyses and DNA affinity precipitation assay (DAPA), this study confirmed the presence of CodY orthologues and corresponding regulons in Gram-negative Synergistetes. A novel palindromic sequence consisting of AT-rich arms separated by a spacer region of variable length and sequence was identified in the promoters of the putative codY-containing operons in Synergistetes. The consensus sequence from genera Synergistes and Cloacibacillus (5′-AATTTTCTTAAAATTTCSCTTGATATTTACAATTTT) contained three AT-rich regions, resulting in two palindromic sequences; one of which is identical to Firmicutes CodY box (5′-AATTTTCWGAAAATT). The function of the consensus sequence was tested by using a recombinant CodY protein (His-CodYDSM) of Cloacibacillus evryensis DSM19522 in DAPA. Mutations in the central AT-rich sequence reduced significantly the binding of His-CodYDSM, whereas mutations in the 5′ or 3′ end AT-rich sequence slightly reduced the binding, indicating that CodYDSM could recognize both palindromic sequences. The proposed binding sequences were found in the promoters of multiple genes involved in amino acids biosynthesis, metabolism, regulation, and stress responses in Synergistetes. Thus, a CodY-like protein from Synergistetes may function similarly to Firmicutes CodY.