A 65-day feeding trial was conducted to investigate the effects of dietary antimicrobial peptide APSH-07 on the growth performance, anti-oxidation responses, stress resistance and intestine microbiota of large yellow croaker Larimichthys crocea. Four isonitrogenous and isolipidic diets were formulated with 0, 30, 60 and 90 mg/kg of APSH-07, respectively. The results showed that the fish fed with 90 mg/kg of dietary APSH-07 had the significantly highest specific growth rate (p < .05). The activities of catalase, superoxide dismutase, the total anti-oxidative capacity and lysozyme had the significantly highest values in liver of fish fed with 90 mg/kg of dietary APSH-07 (p < .05). Serum glucose increased significantly in fish fed diets without APSH-07 supplementation after temperature stress, while serum cortisol increased significantly in those after trawl stress (p < .05). Fish fed with 90 mg/kg of dietary APSH-07 showed significantly higher operational taxonomic units, ACE estimator and phylogenetic diversity whole tree in intestine microbiota compared to fish fed without APSH-07 (p < .05). In conclusion, under the present experimental condition, 90 mg/ kg of antimicrobial peptide APSH-07 supplementation in diet had the better growth performance, higher anti-oxidation and stress resistance capacity, and a potentially more beneficial intestine microbial community of large yellow croaker. K E Y W O R D Santimicrobial peptide, anti-oxidation, growth, intestine microbiota, large yellow croaker, stress