Supershear earthquakes with rupture velocity exceeding shear-wave speeds, previously observed in laboratory experiments and large strike-slip events, often have an initial sub-shear stage before they transition to supershear. In this study, integrated geophysical observations of the 2018 Mw 7.5 Palu, Indonesia earthquake, provide robust evidence of an early and persistent supershear rupture speed. Slowness-enhanced back-projection (SEBP) of teleseismic data provides a sharp image of the rupture process, consistently across multiple arrays. The inferred rupture path agrees with the surface rupture trace inferred from the net surface displacement field derived by sub-pixel InSAR image correlation. The SEBP results indicate a sustained rupture velocity of 4.1 km/s from the rupture initiation to the end, despite large fault bends. The persistent supershear speed is further validated by evidence of far-field Rayleigh Mach waves in regional seismograms. The short or absent supershear transition distance can be caused by high initial shear stress or short critical slip-weakening distance, and promoted by fault roughness near the hypocenter. Steady rupture propagation at a supershear speed considered to be unstable, lower than the Eshelby speed, could result from the presence of a damaged fault zone.

BackgroundFoot-and-mouth disease virus (FMDV) causes a severe vesicular disease in domestic and wild cloven-hoofed animals. Because of the limited early protection induced by current vaccines, emergency antiviral strategies to control the rapid spread of FMD outbreaks are needed.Here we constructed multiple microRNAs (miRNAs) targeting the internal ribosome entry site (IRES) element of FMDV and investigated the effect of IRES-specific miRNAs on FMDV replication in baby hamster kidney (BHK-21) cells and suckling mice.ResultsFour IRES-specific miRNAs significantly reduced enhanced green fluorescent protein (EGFP) expression from IRES-EGFP reporter plasmids, which were used with each miRNA expression plasmid in co-transfection of BHK-21 cells. Furthermore, treatment of BHK-21 cells with Bi-miRNA (a mixture of two miRNA expression plasmids) and Dual-miRNA (a co-cistronic expression plasmid containing two miRNA hairpin structures) induced more efficient and greater inhibition of EGFP expression than did plasmids carrying single miRNA sequences.Stably transformed BHK-21 cells and goat fibroblasts with an integrating IRES-specific Dual-miRNA were generated, and real-time quantitative RT-PCR showed that the Dual-miRNA was able to effectively inhibit the replication of FMDV (except for the Mya98 strain) in the stably transformed BHK-21 cells.The Dual-miRNA plasmid significantly delayed the deaths of suckling mice challenged with 50× and 100× the 50% lethal dose (LD50) of FMDV vaccine strains of three serotypes (O, A and Asia 1), and induced partial/complete protection against the prevalent PanAsia-1 and Mya98 strains of FMDV serotype O.ConclusionThese data demonstrate that IRES-specific miRNAs can significantly inhibit FMDV infection in vitro and in vivo.

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