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Background Large-scale genome reduction has been performed to significantly improve the performance of microbial chassis. Identification of the essential or dispensable genes is pivotal for genome reduction to avoid synthetic lethality. Here, taking Streptomyces as an example, we developed a combinatorial strategy for systematic identification of large and dispensable genomic regions in Streptomyces based on multi-omics approaches. Results Phylogenetic tree analysis revealed that the model strains including S. coelicolor A3(2), S. albus J1074 and S. avermitilis MA-4680 were preferred reference for comparative analysis of candidate genomes. Multiple genome alignment suggested that the Streptomyces genomes embodied highly conserved core region and variable sub-telomeric regions, and may present symmetric or asymmetric structure. Pan-genome and functional genome analyses showed that most conserved genes responsible for the fundamental functions of cell viability were concentrated in the core region and the vast majority of abundant genes were dispersed in the sub-telomeric regions. These results suggested that large-scale deletion can be performed in sub-telomeric regions to greatly streamline the Streptomyces genomes for developing versatile chassis. Conclusions The integrative approach of comparative genomics, functional genomics and pan-genomics can not only be applied to perform a multi-tiered dissection for Streptomyces genomes, but also work as a universal method for systematic analysis of removable regions in other microbial hosts in order to generate more miscellaneous and versatile chassis with minimized genome for drug discovery.

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For the controlled gasoline engine MR479Q, the crank speed, camshaft position, fuel injection, spark ignition timing signals and their relationships under control mode of group ignition and fuel sequential injection were deeply analyzed, then an electronic control unit (ECU) hardware platform solution based on Freescale 16-bit microcontroller MC9S12XEP100 was given out. Taking advantages of the hardware platform itself, a crank event based fuel injection and spark ignition timing control strategy was proposed to enhance traditional fuel injection and ignition reliability. Fuel pulse width, ignition coil dwell time and spark advance control under different engine operating conditions were then designed in detail respectively. The bench test results show that, the fuel injection and spark ignition timing control signals of ECU are accurate and stable enough under steady operating conditions, even under transient operating conditions when step disturbance exists in throttle opening, the fuel pulse width, dwell time and spark advance are also delivered correctly and reliably. The strategy is feasible enough to accomplish precise control of fuel injection and spark ignition.