In the process of civil aircraft production, there will exist such a situation that the fasteners interfere with bend radius and it is hard to replace the structure element because of some limits of objective condition. This article investigates the fatigue quality of structure under tensile load with unloaded fasteners which rivet bucktail overrides bend radius (parallel to load) in civil aircraft by fatigue tests based on DFR method. The results show that the DFR value will not decrease if the fastener interferes with bend radius is not as serious as the situation that the No.6 rivet hole of the structure under tensile load with R5 invases bend radius 1.0mm.