Myocardial injury induced by diabetes has become an increasing health problem. Chrysophanol (CHR) has been widely studied as a potential treatment for many diseases due to its anti-inflammatory effects, but has not been investigated in regard to diabetes-induced myocardial injury. The present study evaluated the myocardial protective effects of CHR in C57BL/KsJ-db/db diabetic mice. C57BL/KsJ-db/db and C57BLKS/J mice were treated with vehicle, metformin (100 mg/kg/day) or CHR (50 or 100 mg/kg/day) for 28 days. An oral glucose tolerance test was performed to detect blood glucose levels. Blood lipids, triglycerides, total cholesterol, myocardial function-associated enzymes, namely creatine kinase (CK) and lactate dehydrogenase (LDH), and insulin levels were analyzed. TNF-α, interleukin (IL)-1β and IL-6 inflammatory cytokine levels in serum and myocardial tissues were determined by ELISA. Expression of silent information regulator l (SIRT1) and high mobility group box 1/NF-κB pathway-associated proteins in myocardial tissues were measured by western blot analysis and immunohistochemistry. CHR treatment at both concentrations markedly decreased blood lipid and serum insulin levels, and inhibited the myocardial enzymes CK and LDH. CHR also significantly ameliorated the cardiac pathological changes in diabetic mice. The inflammatory cytokine levels that were increased in C57BL/KsJ-db/db diabetic mice were downregulated by CHR treatment. CHR also increased SIRT1 protein expression and inhibited activation of the HMGB1/NF-κB pathway. In conclusion, the present study indicates that CHR effectively protected against diabetic myocardial injury via regulation of SIRT1 and the HMGB1/NF-κB signaling pathway.

: Tissue plasminogen activator (tPA) is commonly used to treat acute ischemic stroke within an appropriate therapeutic window. Its inhibitor, neuroserpin (NSP), is reported to exhibit neuroprotective effects on stroke. This review aims to summarize, from literature, the available evidence, potential mechanisms, and knowledge limitations regarding the neuroprotective role of NSP in stroke. All the available evidence indicates that regulation of the inflammatory response may play a key role in the mechanisms of NSP, which involve all the constituents of the neuroimmune axis. The neuroinflammatory response triggered by stroke can be reversed by NSP, with complicated mechanisms such as maintenance and reconstruction of the structure and function of the blood–brain barrier (BBB), protection of the cells in the central nervous system, and suppression of cell death in both ischemic and hemorrhagic stroke. Moreover, available evidence strongly suggests a tPAindependent mechanism is involved in NSP. However, there are many important issues that are still unclear and need further investigation, such as the effects of NSP on hemorrhagic stroke, the role of the tPA-independent neuroprotective mechanisms, and the clinical application prospects of NSP. We believe our work will be helpful to further understand the neuroprotective role of NSP.

Objective: To explore the effect of functional exercise nursing plan based on evidence-based nursing in patients after hip replacement. Methods: A total of 150 patients who underwent hip replacement in the orthopedic second ward of our hospital during the research period from January 2010 to January 2020 were selected as the research object. The random number expression method was applied to achieve grouping of patients. Among them, the control group received conventional rehabilitation treatment; the experimental group guided rehabilitation exercises according to the functional exercise nursing plan after hip replacement, and the rehabilitation conditions of the two groups were compared. Results: Compared with the patients in the control group, the bed time (7.45 ± 2.10) days and average hospital stay (15.84 ± 3.29) of the experimental group were relatively shorter. The incidence of complications in the experimental group and the control group was 6.67% and 16.00%, which are significantly different. The difference in Harris hip score before surgery was small, and the difference when discharge and after discharge was gradually increased, showing a better care effect in the experimental group. There was no significant difference in preoperative quality of life scores between the two groups before surgery (P > 0.05). The life quality of the experimental group was significantly higher than that of the control group at discharge, 1 month after discharge and 3 months after discharge, and showed most significantly after 3 months. Conclusion: The effect of functional exercise nursing plan based on evidence-based nursing in patients after hip replacement is remarkable, which can promote the recovery of patients, improve the quality of life, reduce the hospitalization time, reduce the incidence of postoperative complications, and has a positive significance in promoting clinical development in China.