Our recent development of a wireless humidity sensor system embedded in incontinence products enables new sensor applications to diagnose and supervise geriatric diseases (i.e., age-related diabetes mellitus type II). The measurement of glucose in urine, so-called glucosuria, is an early indicator for an incipient diabetes mellitus disease, whose symptoms are often age-related but misjudged. In this paper, an incontinence glucose sensor is printed with biocompatible ink and Prussian blue as an electron mediator on foil and functionalized with immobilized glucose oxidase. Inkjet printing of multiple layers of Nafion prevents large interference substances from diffusing into the measuring electrode and allows precise adjustment of the linear working range, which is significantly different from blood glucose measurement. Performance tests show the potential to detect minimum glucose values and store the sensor over a prolonged period at room temperature. The printed glucose sensor can be embedded into the absorber material of incontinence products, where capillary forces transport the urine analyte to the detection area. An attached readout module with an integrated potentiostat measures the glucose concentration in urine, which is transmitted wirelessly with incontinence events and stored in a cloud service for further analysis by medical staff and care workers.

The fast paced development in life sciences in recent years resulted in the integration of laboratory equipment into miniaturized mobile point-of-care diagnostic units as well as biotechnological microfluidic systems. Similar to the evolution of microelectronics, the "Micro System Technology" is the driver for the integration and miniaturization on the system level. For PoC, this clearly is reflected in the increased functionality, the number of analysis performed per devices and the reduced amount of sample liquid required to run a high-quality analysis.Due to the physical constraints, however, this evolution is not easily adopted to the optical analytics, like microscopy. As neither the lightpath nor the lens sizes can be modified without a complete re-engineering of the system, the evolution has stalled here. Therefore, the presented work targets the development and implementation of a lens-less microscope combining optics and microfluidics specifically for cell and particle analysis. Conceptually designed without any movable elements or lenses, the images obtained can be processed digitally for analysis and visualization. This is achieved by combining modern image sensors, holography principles and image deconvolution to enable novel approaches to microscopic observations, be it bacteria, cell clusters or micro particles.We show in this work the path to realization for the lens less microscope from the initial concept, the implementation strategy to demonstrating the capabilities of the device.The innovation is compared to the capabilities of conventional microscopy, using both live organisms and particles as well as simulation models to holography imaging as well as photonic wave theory.The microscope platform is demonstrated as a 3D rapid prototype, offering a versatile system for easy implementation in a laboratory and research setting.Microscopic sampling is used to offer correlation of the actual achievements of the system and derive future optimization criteria. Also, the viability w.r.t. cytometrics and live observation in microfluidic bioreactors id demonstrated.A synopsis on the achievements as well as the outlook w.r..t. fluorescence or tomography analysis is given.

Novel approaches in cell based and cell free micro reactors promise a new generation of high quality, high purity and on top personalized synthesis of drugs like antibiotics and functional proteins. These concepts are - however-relying on very specific conditions, under which the cells need to work and therefore require advanced sensing for e.g. ionic content, pH value and temperature. Also, the creation of product itself needs to be monitored to extract the valuable drug from the reactor, before side reactions start to deteriorate quality and yield. Microfluidics has come a long way since, resulting in advanced Point of Care devices nowadays, which can run complex protocols/1/. However, these reactions are time based and the process conditions are fixed and not monitored at all. To leverage the capability to drug production, monitoring sensors need to be integrated into the fluidic system, creating a complex electronic-optical microfluidic device. Exemplary techniques for the integration of sensors are provided in this paper, technological approaches and experimental results are given