Introduction: The confirmation of adenovirus infection using Real time PCR (qPCR) afford a greater accuracy, with a specific, sensitive, quantitative technique as well as time consuming. Adenovirus infections can be detected using a variety of techniques, although the most accurate ones are molecular ones. Method: This study includes 90 patient who suffering from  respiratory symptoms (common colds) and/or diarrhea with high temperature, their ages range from newborn to elderly, who are inpatients and outpatients resident in Fallujah Maternity and Children's Hospital and teaching hospital in Fallujah / Anbar governorate, from 1st November 2021 to 1st May 2022. All samples subjected to Adenovirus identification using Real Time PCR Test. Results: Out of total 90 blood sample there were 12(13.3%), 78(86.7%) Negative and positive results respectively, from Real Time PCR results.