BackGround: Adolescents self-care practices and hygienic care are a critical issues that determines the health status of them. This study aimed to assess the preventive self-care practices against urinary tract infection (UTI) among secondary nursing students. A cross sectional descriptive design was adopted. All secondary nursing schools in Port-Said Governorate were the setting. A total of 247of female nursing students were recruited suing simple random sampling technique. An interviewing Questionnaire sheet was used to assess students' self-care practices regarding prevention of UTI. The results revealed that 72.1% of girls had poor level of knowledge regarding anatomy and physiology of the urinary system and urinary tract infection. While, less than two-thirds of them had inadequate total practices. The results showed that, strong positive correlation was detected between students' total knowledge score and total practice score. Conclusion:Majority of nursing students scored poor level of knowledge regarding UTIs and its preventive hygienic practices. Recommendations: more attention should be given to establish educational strategies towards improving female students' health awareness in this topic.The secondary nursing education is believed the largest source of nursing graduates, providing about 94% of the available nursing workforce. More than that, those nurses are very young, ranging from mid-teenage to young adulthood (El-Noshokarty, 2004).Self -care is the development and use of personal health practices and effective ways to deal with problems and stress for increase their own health, preventing or limiting disease, and maintains well-being.These activities are usually tried without other help. However; in spite of its great importance of self -care practices for health maintenance it may be harmful if it is based on wrong knowledge or harmful attitudes (Mazor-Dray., Levy, Schlaeffer et al; 2011).Community nurses play an extremely important role central to public education on health hygiene and personal development, including the maintenance of best menstrual and self-care practices among female teens. They always come in contact with this capable of being hurt group and therefore, it is necessary for them to have good knowledge as well as becoming role models to this deliquescent group of the society. It this way becomes as a role to secure/make sure of proper education and encouragement of personal cleaning and best self -care practices among female teens in our community (Adika, Ayinde et al., 2013).Assess the preventive knowledge and self-care practices against urinary tract infection (UTI) among secondary nursing students. Research objectives1. Assessing secondary nursing student's knowledge regarding urinary tract infections.2. Identifying self-care practices regarding prevention of urinary tract infection among secondary nursing students. Research QuestionsTo achieve this aim, the following research questions were formulated:1. What are the levels of secondary nursing student's knowledge regard...