BackgroundThe prevalence of resistance to fusidic acid of methicillin-resistant Staphylococcus aureus (MRSA) was increased each year in a Taiwan hospital. Thirty-four MRSA clinical isolates collected in 2007 and 2008 with reduced susceptibility to FA were selected for further evaluation the presence of resistance determinants.ResultsThe most common resistance determinant was fusC, found in 25 of the 34 MRSA isolates. One of the 25 fusidic acid-resistant MRSA harboured both fusB and fusC, which is the first time this has been identified. Mutations in fusA were found in 10 strains, a total of 3 amino-acid substitutions in EF-G (fusA gene) were detected. Two substitutions with G556S and R659L were identified for the first time. Low-level resistance to fusidic acid (MICs, ≤ 32 μg/ml) was found in most our collection. All collected isolates carried type III SCCmec elements. MLST showed the isolates were MRSA ST239. PFGE revealed nine different pulsotypes in one cluster.ConclusionsOur results indicate that the increase in the number of fusidic acid resistant among the MRSA isolates in this hospital is due mainly to the distribution of fusC determinants. Moreover, more than one fusidic acid-resistance mechanism was first detected in a same stain in our collection.

Dust plays an important role in climate changes as it can alter atmospheric circulation, and global biogeochemical and hydrologic cycling. Many studies have investigated the relationship between dust and temperature in an attempt to predict whether global warming in coming decades to centuries can result in a less or more dusty future. However, dust and temperature changes have rarely been simultaneously reconstructed in the same record. Here we present a 1600-yr-long quantitative record of temperature and dust activity inferred simultaneously from varved Kusai Lake sediments in the northern Qinghai–Tibetan Plateau, NW China. At decadal time scale, our temperature reconstructions are generally in agreement with tree-ring records from Karakorum of Pakistan, and temperature reconstructions of China and North Hemisphere based on compilations of proxy records. A less or more dusty future depends on temperature variations in the Northern Qinghai-Tibetan Plateau, i.e. weak and strong dust activities at centennial time scales are well correlated with low and high June–July–August temperature (average JJA temperature), respectively. This correlation means that stronger summer and winter monsoon should occur at the same times in the northern Qinghai–Tibetan Plateau.

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