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Two feature selection techniques and a multilayer perceptron (MLP) neural network (NN) have been used in this study for human chromosome classification. The first technique is the "knock-out" algorithm and the second is the Principal Component Analysis (PCA). The "knock-out" algorithm emphasized the significance of the centrometric index and of the chromosome length, as features in chromosome classification. The PCA technique demonstrated the importance of retaining most of the image information whenever small training sets are used. However, the use of large training sets enables considerable data compression. Both techniques yield the benefit of using only about 70% of the available features to get almost the ultimate classification performance.

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