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Summary. Tbe production of baemoRlobin C (Hb-C) wa.s examined in tbree sheep and two goats wbicb were kept anaemic by bleeding for 5-7 weeks. A fourtb sbeep was made acutely anaemic Ijy bleeding severely for tbree days.In the chronically bled sheep the production of Hb-C wa.s variable and difficult to maintain unless the animals were made severely anaemic.In tbe goats tbe replacement of the ^ chain was almost complete witb an anaemia wbicb was equivalent to tbat produced in tbe sbeep. and Hb-C could be maintained for a considerable period.In the acutely bled sbeep Hb-C increased to a maximum by tbe 16th day, ReticulocytosLS was not marked, but, in general, an increase occurred about 15 days before maximum Hb-C production was evident.Erytbropoietin activity was examined in four animals witb variable results, bnt in the severely bled animal it was evident before tbe reticulocytes appeared. Tbis activity did not appear to he related to tbe amount of Hb-C whicii was produced.The elimination of Hb-C from tbe (irculation was very variable, but tbe rates of decrease in goats were slower than in sbeep.INTRODUCTION.

The DEAE-cellulose chromatographic procedure which uses glycine-KCN-NaCl solutions as Developers and a NaCl gradient to elute the hemoglobin zones was found to be useful for the quantitation of Hb F in samples containing Hb A, provided the level of Hb F exceeds 2 percent. The method has been evaluted through a study of artificial mixtures and of blood samples from patients with various disorders. The data have been compared with results obtained by four other procedures that are used for the quantitation of Hb F.

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