RP-HPLC ultraviolet detection simultaneous quantification of piracetam and levetiracetam has been developed and validated. The chromatography was obtained on a Nucleosil C18 column of 25 cm × 0.46 cm, 10 μm, dimension. The mobile phase was a (70 : 30 v/v) mixture of 0.1 g/L of triethylamine and acetonitrile. Smooth flow of mobile phase at 1 mL/min was set and 205 nm wavelength was selected. Results were evaluated through statistical parameters which qualify the method reproducibility and selectivity for the quantification of piracetam, levetiracetam, and their impurities hence proving stability-indicating properties. The proposed method is significantly important, permitting the separation of the main constituent piracetam from levetiracetam. Linear behavior was observed between 20 ng/mL and 10000 ng/mL for both drugs. The proposed method was checked in bulk drugs, dosage formulations, physiological condition, and clinical investigations and excellent outcome was witnessed.

This review article recapitulates the analytical methods for the quantitative determinations of diltiazem and three H2receptor antagonists (cimetidine, ranitidine, and famotidine) by one of the spectroscopic technique (UV spectrophotometery) and separation technique such as high-performance liquid chromatography (HPLC). The clinical and pharmaceutical analysis of these drugs requires effective analytical procedures for quality control, pharmaceutical dosage formulations, and biological fluids. An extensive survey of the literature published in various analytical and pharmaceutical chemistry-related journals has been compiled in its review. A synopsis of reported spectrophotometric and high-performance liquid chromatographic methods for individual drug is integrated. This appraisal illustrates that majority of the HPLC methods reviewed are based on the quantitative analysis of drugs in biological fluids, and they are appropriate for therapeutic drug monitoring purpose.

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This research introduces an RP-HPLC method to estimate dorzolamide and timolol maleate quantitatively in the simultaneous mode. The stationary phase has consisted of A phenomenix® C18 column which gave satisfactory separation of these drugs when subjected to analysis, where in the mobile phase is composed of Acetonitrile:Buffer (20:80,v/v). The instrument was set up as such that the pump draws mobile phase at a flow rate of 1.0 mL/min and monitored at 290nm wavelength. Linear relationship of concentration of drugs vs. peak area was ascertained to assess linearity parameters and a correlation coefficient of more than 0.9995 for all the drugs was achieved when studied in a range of 10 to 1000 ug/mL. The newly developed method showed high accuracy and precision with a percent recovery of 98.85% -101.23% and a percent relief standard deviation of 0.5 -1.7 %. The optimized method was validated according to the guideline laid down by ICH and was successfully applied to quantitate both the drugs in bulk, raw material, eye drops formulation and in physiological fluids.

The current article described a completely validated RP-HPLC method that described simultaneous quantification of cetirizine in the presence of any of the three cephalosporins, i.e., ceftriaxone or cephalexin or cefotaxime while amlodipine (calcium channel blocker) used as an internal standard. Cetirizine, a widely prescribed drug as allergy medication. It is also co-administered with cephalosporins. The methanol: acetonitrile: water in a ratio of 65:5:30 with a pH to 3.2 with phosphoric acid mobile phase was selected to keep the method least expensive. All the validation parameters such as accuracy, precision, repeatability, suitability were checked and were found to be friendly according to ICH guidelines. The LOD and LOQ in each case were evaluated and for cetirizine were found to be 0.001 and 0.003 µgmL -1 , ceftriaxone 0.06 and 0.211 µgmL -1 , cephalexin 0.016 and 0.054 µgmL -1 , cefotaxime 0.012 and 0.04 µgmL -1 , respectively. The method was found to possess applications for clinical purposes and pharmaceutical labs.