<p class="Abstract">Epilepsy is an abnormality of nervous system showing seizures. Despite, the known antiepileptic activity of plants possessing anti-oxidant properties, <em>Silybum marianum</em> has not been studied yet. Therefore, present experiment was planned to evaluate antiepileptic potential of <em>S. marianum</em> (100, 200 and 300 mg/kg) seeds ethanol extract in pentylenetetrazol-induced kindled mice (n = 30). <em>S. marianum</em> seed extract (300 mg/kg) provided significant protection against pentylenetetrazol-induced convulsions (seizure intensity, latency and lethality) comparable with reference drug (valproic acid). Furthermore, 300 mg/kg/day dose was effective to prevent oxidative stress causing significant decrease in the lipid peroxidation (1.4 ± 0.4 nmol/mg protein) and increased superoxide dismutase (0.4 ± 0.1 μmol/mg protein) and catalase activity (4.7 ± 0.8 U/mL) of mice brain as compared to induced untreated group (p&lt;0.05). It was concluded that antiepileptic activity of <em>S. marianum</em> seeds was due to its anti-oxidant property.</p><p class="Abstract"><strong>Video Clip</strong></p><p class="Abstract"><a href="https://www.youtube.com/v/NAh_04UzpYM">Experimental induction of seizures:</a> 10 min 4 sec</p><p> </p>