Most of the commercial papaya genotypes show susceptibility to water deficit stress and require high volumes of irrigation water to yield properly. To tackle this problem, we have collected wild native genotypes of Carica papaya that have proved to show better physiological performance under water deficit stress than the commercial cultivar grown in Mexico. In the present study, plants from a wild Carica papaya genotype and a commercial genotype were subjected to water deficit stress (WDS), and their response was characterized in physiological and molecular terms. The physiological parameters measured (water potential, photosynthesis, Fv/Fm and electrolyte leakage) confirmed that the papaya wild genotype showed better physiological responses than the commercial one when exposed to WDS. Subsequently, RNA-Seq was performed for 4 cDNA libraries in both genotypes (susceptible and tolerant) under well-watered conditions, and when they were subjected to WDS for 14 days. Consistently, differential expression analysis revealed that after 14 days of WDS, the wild tolerant genotype had a higher number of up-regulated genes, and a higher number of transcription factors (TF) that were differentially expressed in response to WDS, than the commercial genotype. Thus, six TF genes (CpHSF, CpMYB, CpNAC, CpNFY-A, CpERF and CpWRKY) were selected for further qRT-PCR analysis as they were highly expressed in response to WDS in the wild papaya genotype. qRT-PCR results confirmed that the wild genotype had higher expression levels (REL) in all 6 TF genes than the commercial genotype. Our transcriptomic analysis should help to unravel candidate genes that may be useful in the development of new drought-tolerant cultivars of this important tropical crop.

Stingless bees are ecologically and economically important species in the tropics and subtropics, but there has been little research on the characterization of detoxification systems and immune responses within them. This is critical for understanding their responses to, and defenses against, a variety of environmental stresses, including agrochemicals. Therefore, we studied the detoxification and immune responses of a stingless bee, Nanotrigona perilampoides, which is an important stingless bee that is widely distributed throughout Mexico, including urban areas, and has the potential to be used in commercial pollination. We first determined the LC50 of the neonicotinoid insecticide imidacloprid for foragers of N. perilampoides, then chronically exposed bees for 10 days to imidacloprid at two field-realistic concentrations, LC10 (0.45 ng/µL) or LC20 (0.74 ng/µL), which are respectively 2.7 and 1.3-fold lower than the residues of imidacloprid that have been found in honey (6 ng/g) in central Mexico. We found that exposing N. perilampoides stingless bees to imidacloprid at these concentrations markedly reduced bee survival and food consumption, revealing the great sensitivity of this stingless bee to the insecticide in comparison to honey bees. The expression of detoxification (GSTD1) and immune-related genes (abaecin, defensin1, and hymenopteacin) in N. perilampoides also changed over time in response to imidacloprid. Gene expression was always lower in bees after 8 days of exposure to imidacloprid (LC10 or LC20) than it was after 4 days. Our results demonstrate that N. perilampoides stingless bees are extremely sensitive to imidacloprid, even at low concentrations, and provide greater insight into how stingless bees respond to pesticide toxicity. This is the first study of its kind to look at detoxification systems and immune responses in Mexican stingless bees, an ecologically and economically important taxon.

Nutrition is vital for health and immune function in honey bees (Apis mellifera). The effect of diets enriched with bee-associated yeasts and essential oils of Mexican oregano (Lippia graveolens) was tested on survival, food intake, accumulated fat body tissue, and gene expression of vitellogenin (Vg), prophenoloxidase (proPO) and glucose oxidase (GOx) in newly emerged worker bees. The enriched diets were provided to bees under the premise that supplementation with yeasts or essential oils can enhance health variables and the expression of genes related to immune function in worker bees. Based on a standard pollen substitute, used as a control diet, enriched diets were formulated, five with added bee-associated yeasts (Starmerella bombicola, Starmerella etchellsii, Starmerella bombicola 2, Zygosaccharomyces mellis, and the brewers’ yeast Saccharomyces cerevisiae) and three with added essential oils from L. graveolens (carvacrol, thymol, and sesquiterpenes). Groups of bees were fed one of the diets for 9 or 12 days. Survival probability was similar in the yeast and essential oils treatments in relation to the control, but median survival was lower in the carvacrol and sesquiterpenes treatments. Food intake was higher in all the yeast treatments than in the control. Fat body percentage in individual bees was slightly lower in all treatments than in the control, with significant decreases in the thymol and carvacrol treatments. Expression of the genes Vg, proPO, and GOx was minimally affected by the yeast treatments but was adversely affected by the carvacrol and thymol treatments.

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