Traffic simulation is widely used for modeling, planning, and analyzing different strategies for traffic control and road development in a cost-efficient manner. In order to perform an intersection simulation, random vehicle trip data are typically applied to an intersection network, making them unrealistic. In this paper, we address this issue by presenting two different methods of incorporating actual turning movement count (TMC) data and comparing their similarity for intersection simulation and analysis. The TMC of three intersections in Las Vegas are estimated separately for one hour using a developed vision-based tracking system and they are incorporated into Simulation of Urban MObility (SUMO) for estimating traffic measurements and traffic signal design. t-tests with a 95% confidence interval on the simulation variables demonstrate the importance of using a route-based creation method which injects vehicles into a simulation environment based on the frame-level departure time. The intersection analyses and comparisons are performed based on estimated traffic measurements such as travel time, density, lane density, occupancy, and normalized waiting time. Since the critical edge of each intersection network is identified based on a higher normalized waiting time, new traffic signal designs are suggested based on the actual critical turning movements and improvements in vehicle travel time are achieved to better accommodate the actual traffic demand.