Optical limiting performances in Fluorescein with different concentration of 2, 4, 6 and 8 mM are investigated by using 473 nm continuous wave (cw) laser. The optical limiting behavior is investigated via transmission measurement through the sample at different concentrations. The investigation shows that the optical limiting capability is concentration dependent. The results showed that the sample has obvious optical limiting effect. 8 mM concentration has the best limiting effect among the four concentrations chosen. It is also found that the threshold value of optical limiting is affected by sample absorption coefficient. The Fluorescein exhibits good optical limiting properties in solution.