This study aimed to examine the cytotoxic effect of resveratrol as an anticancer in human colorectal cancer (HRT) cell line by assessment of its half-maximal inhibitory concentration (IC50) and its ability to inhibit the growth of these cancerous cells. Resveratrol inhibited the proliferation of HRT cell lines when used at different increased concentrations in this study (25, 50, 100, 200, 300) μmol respectively. These increased concentrations of resveratrol caused a corresponding significant inhibition in the growth percentage of the tested cancerous cell line (13%, 31.33%, 53.66%, 63.66 %, and 76.33%) respectively when compared with DMSO0.1% as negative control, in a concentration-dependent manner. Resveratrol at 300 μmol concentration showed the highest significant increase in the growth inhibitory percentage (76.33%). Moreover, resveratrol IC50 against (HRT) cell line was determined as 75.63 μmol. The study suggested a promising anticancer activity of resveratrol which can interfere with many dysregulated signaling pathways in transformed cells which are proposed to be driving forces for its anticancer effect.

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The experimental studies of Aluminum Phosphide (AP) poisoning in rats revealed several clinical and pathological signs such as hemorrhage, sinusoidal dilatation, bile stasis, centrilobular necrosis, Kupffer cell hyperplasia, infiltration by mononuclear cells, and fatty infiltration in the liver tissues. This paper aimed to show the impact of carrots on the toxic effect of AP on the livers of adult rats (female). To investigate some biochemical and histopathological changes effects of AP in rats, sixty white female rats were equally divided into four groups, the first group (G1) was administered orally with 3mg/kg/ body weight of AP, the second group (G2) was orally treated with AP and 10% carrot extract at the same time. The third group (G3) administrated 10% carrot extract only. The fourth (G4) group was the negative control and was treated with distilled water only. The experiments continued for a month at the animal house of the Veterinary Medicine College of Baghdad University. The results revealed that high levels of liver enzymes and bilirubin were induced in G1 with decreasing total protein levels. The pathological examination revealed the presence of marked proliferation of Kupffer cells in G1 livers. However, the G2 group showed slight infiltration of lymphocytes in sinusoids. The pathological changes in the livers of G3 group showed slight cloudy swelling in hepatocytes compared with the normal texture of hepatocytes in G4. The data of this experiment showed that treatment with carrot extract significantly decreases the elevation in the level of liver function enzymes in animal poisoned with AP. In addition, treatment with carrot extract reduces the severe damage in the hepatic tissue that occurred in rats treated with AP only. In general, it could be concluded that treatment with carrot extract provides a remedial effect against the hepatotoxicity that is resulted from exposure to AP.

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