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Nowadays herbal medicine presents a significant adjuvant tool for hard treatment, especially in the case of cancer where modern medicine has access to traditional medicine to deprive the patient of the side effects of therapeutic approaches such as surgery and chemotherapy. Thus, Lebanese 10452 km 2 are so rich in medicinal plants such as Eryngium creticum L. and Euphorbia macroclada Boiss that are traditionally used in the treatment of various diseases (leukemia, asthma, skin diseases, antidote to snake venom, tumors, etc.). To fully realize the importance of these two Lebanese plants, we tried at first to study the phytochemistry of three extracts (aqueous, methanolic and ethyl acetate) from fresh leaves and stems of both plants. In a second step, the antioxidant capacity of these three extracts from the two parts of the fresh plants using spectrophotometric analysis has been evaluated and their cytotoxicity on the MCF7 breast cancer cell line by the XTT Cell viability technique has been studied. Our results showed that both leaves and stems of these two plants contain alkaloid, tannin, coumarin, saponin, flavonoid, polyphenol and reducing sugars in different concentrations. Moreover, both leaves and stems have exerted an antioxidant activity that may be due to their phenolic content and they have also inhibited the growth of cancer cell line from 68 % to 72 %. These results showed that both plants can be considered as a good source of natural products that can be used in the prevention of several diseases.

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Objective: The objective of this study is to evaluate the chemical contents (total phenolic and total flavonoid contents) of the crude ethanolic and aqueous extracts from leaves and stems of a Lebanese plant and to evaluate their cytotoxic and antioxidant activities.Methods: Quantification of the total phenolic and total flavonoid contents of all extracts of Anacyclus nigellifolius from leaves and stems and the cytotoxic activity were evaluated through XTT assay, while antioxidant activities were evaluated using spectrophotometric analyses DPPH, H 2 O 2 , and chelating of ferrous ions tests. Results:The ethanolic extract from leaves and stem of A. nigellifolius was better than aqueous extract and showed a higher content in total phenolic and total flavonoid which was 39.48±0.04 and 13±0.03, respectively. On the other hand, the best value of IC50 of DPPH, H 2 O 2 , and chelating of ferrous ions of ethanol extract from leaves was 0.1 mg/mL, 0.25 mg/mL, and 0.37 mg/mL, respectively, also, ethanol extract has significantly inhibited cells growth 293T and MCF7, a percentage of inhibition was in leaves, and it has reached the 70% and 54%, respectively. Conclusions:Both ethanolic and aqueous extracts of both parts of A. nigellifolius have shown high efficiency and good antioxidant and antiproliferation power. Hence, this plant can be used in the prevention of a number of diseases related to oxidative stress as well as might be used for inhibition of cancer cell line growth.

Psophocarpus tetragonolobus has long been used in traditional medicine and cuisine. In this study, Psophocarpus tetragonolobus extracts were isolated by maceration and ultrasound-assisted extraction and were evaluated for their antioxidant and anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. The obtained results show that both extracts (maceration and ultrasound) were rich in bioactive molecules and exerted substantial antioxidant and anti-inflammatory effects. The P. tetragonolobus extracts’ treatment in LPS-stimulated RAW264.7 macrophages resulted in a significant downregulation of the expressions of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β mRNA. In addition, the P. tetragonolobus extracts’ treatment attenuated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression. Our observations indicate that there is no significant difference between the two studied extracts of P. tetragonolobus in terms of biological properties (specifically, antioxidant and anti-inflammatory effects. Regardless of the extraction method, P. tetragonolobus could be used for treating diseases related to oxidative stress and inflammatory reactions.