Enzyme pectinase dạng bột dễ vận chuyển và bảo quản. Vì vậy, nghiên cứu đã sử dụng enzyme pectinase hoạt tính 194 UI/mL từ Aspergillus niger để tạo chế phẩm dạng bột bằng phương pháp sấy phun (nồng độ chất trợ sấy maltodextrin 10% (w/v), nhiệt độ sấy 130°C, tốc độ nhập liệu 288 mL/h). Bên cạnh đó, nghiên cứu đã xác định được thông số động học của chế phẩm enzyme Km là 28,4 mg/mL, pH tối ưu 5,0, nhiệt độ tối ưu 40°C, Cu2+ là chất hoạt hóa và Ag+ là chất kìm hãm hoạt động enzyme.

This study used polysaccharide (PS) from yeast cell walls to encapsulate polyphenols (PP) extracted from green tea (a good antioxidant substance) to avoid the effects of sensitive factors such as temperature, light and oxygen, and to preserve its stability and bioactivities. The objective of this study was to investigate the effects of four types of wall material (PS-MD (maltodextrin), PS-whey, whey, and MD). After selecting the most appropriate wall material, the study continued to investigate the effects of the ratios of the core to the wall material (1:1, 1:2, 1:3 and 1:4, w/w); the inlet air temperatures of spray-drying (130, 140, 150 and 160 °C); and the feed flow rates (04, 05, 06 and 07mL/min) on the microencapsulation yield (MEY) and the microencapsulation efficiency (MEE). The results showed the best settings as follows the wall material being PS-MD, the ratio of PP to the wall material as 1:3 (w/w), the inlet air temperatures at 140 °C, and the feed flow rate at 05mL/min. Under those conditions, the MEY and the MEE were found to be highest as 31.118mg/g and 86.73%, respectively. When examined by scanning electron microscopy (SEM), they had various sizes and spherical shapes and some of them had smooth or concave surfaces. As a result, it could be concluded that polyphenols were successfully encapsulated in the PS-MD matrix by spray-drying and the application to some food products could be further studied.

Ganoderma lucidum is a rare medicinal mushroom that is very valuable in the prevention and fight against diseases, especially the prevention against cancer, enhancing the immune system of the body, respiratory system, cardiovascular system, digestive system, secretion, help extend life,… Substrate for growing Ganoderma lucidum are mainly rubber sawdust from the provinces of Dong Nai, Tay Nguyen in Việt Nam. The use of peanut stem leaves peanut, peanut shell has solved the environmental pollution, helping to utilize the excess waste and add more substance into mushroom Ganoderma lucidum growing.