Antibody phage display provides a powerful and efficient tool for the discovery and development of monoclonal antibodies for therapeutic and other applications. Antibody clones from synthetic libraries with optimized design features have several distinct advantages that include high stability, high levels of expression, and ease of downstream optimization and engineering. In this study, a fully synthetic human scFv library with six diversified CDRs was constructed by polymerase chain reaction assembly of overlapping oligonucleotides. In order to maximize the functional diversity of the library, a beta-lactamase selection strategy was employed in which the assembled scFv gene repertoire was fused to the 5'-end of the beta-lactamase gene, and in-frame scFv clones were enriched by carbenicillin selection. A final library with an estimated total diversity of 7.6 x 10(9), greater than 70% functional diversity, and diversification of all six CDRs was obtained after insertion of fully randomized CDR-H3 sequences into this proofread repertoire. The performance of the library was validated using a number of target antigens, against which multiple unique scFv sequences with dissociation constants in the nanomolar range were isolated.

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SUMMARYDuring the prenatal period, development of individual is influenced by the environmental factors. In mammals, 5hydroxytryptamine (5HT) is a central neurotransmitter that regulates behavioral functions in the vertebrate brain. Tryptophan hydroxylase (TPH) is a rate-limiting enzyme in the 5-HT synthesis. Various stresses are known to modulate the 5-HT synthesis and the TPH expression. In the present study, the influence of prenatal noise and music application on the 5-HT synthesis and the TPH expression in the dorsal and median raphe of young rats was investigated. Prenatal noise application increased the 5-HT synthesis and the TPH expression in the dorsal and median raphe nuclei of offspring. Prenatal music application, in contrast, decreased the 5-HT synthesis and the TPH expression in the dorsal and median raphe nuclei of offspring. The present study suggests that prenatal stimuli including noise and music may affect the emotional state of offspring.

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