We aimed to evaluate the protective effects of Yuk-Mi-Jihwang-Tang (YJT) against acute restraint stress-induced brain oxidative damage. A water extract of YJT was prepared and subjected to high performance liquid chromatography - diode array detector-mass spectrometry (HPLC-DAD-MS). Thirty-six heads of C57BL/6J male mice (7 weeks) were divided into six groups (n = 6/group). The mice were orally administrated YJT (0, 50, 100, or 200 mg/kg) or vitamin C (100 mg/kg) for 5 consecutive days before 6 h of acute restraint stress. In the brain tissue, lipidperoxidation, antioxidant components, and pro-inflammatory cytokines were measured, and the serum corticosterone level was determined. Acute restraint stress-induced notably increased lipid peroxidation in brain tissues, and pretreatment with YJT showed a significant decreased the lipid peroxidation levels (p< 0.05). The levels of antioxidant components including total glutathione contents, activities of SOD and catalase were remarkably depleted by acute restraint stress, whereas these alterations were significantly restored by treatment with YJT (p< 0.05 or p< 0.01). The restraint stress markedly increased pro-inflammatory cytokines, such as TNF-α and IL-6 in the gene expression and protein levels (p< 0.05 or p< 0.01). Pretreatment with YJT significantly attenuated serum corticosterone (200 mg/kg, p < 0.05). YJT drastically attenuated the levels of 4- HNE, HO-1, Nox 2 and iNOSwhich were elevated during acute restraint stress, whereas the Nrf2 level was increased in brain tissue protein levels. Our data suggest that YJT protects the brain tissue against oxidative damage and regulates stress hormones.

Purpose: This study was carried out to investigate antioxidant activities of Sunsik supplemented with cultivated wild ginseng powder and its applicability as a beauty food. Methods: After obtaining extracts from cultivated wild ginseng powder and Sunsik using 70% ethanol, the antioxidant activity of Sunsik supplemented with cultivated wild ginseng powder was measured using the radical scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH). The contents of calcium, phosphorus, magnesium, potassium, and sodium were also measured by an inductively coupled plasma method. The mean and standard deviation were calculated using SPSS statistics 21. ANOVA was performed for each test group and Duncan's multiple range test was performed at the significance level of 5%. Results: Total polyphenol contents of Sunsik with 0.6% 15-year cultivated wild ginseng powder (15yrWS0.6) were determined to be the highest one in all the samples, as was the DPPH radical scavenging activity of 15yrWS0.6. Conclusion: Therefore, this study demonstrates that Sunsik possesses antioxidant activity, suggesting that supplemented with cultivated wild ginseng powder can act as an effective functional inner beauty food for health.

Posters 642 ill at home. Analysis followed the tenets of Glaserian grounded theory methodology (Glaser 1978; 1992) until theoretical saturation was reached.