This study examined the effectiveness of a new empathy-oriented prototyping toolkit that was carefully developed to aid empathy gaining in students who find it difficult to express their ideas using only traditional paper-based prototyping materials. The toolkit has a simple structure and uses basic geometric three-dimensional forms made of various surface materials, such as rubber, wood, metal, acrylic and marble, which evoke distinct tactile sensations. The toolkit substitutes the items used in paper-based prototyping, such as construction paper, glue, scissors, paper cups and permanent markers, in the prototyping stage of the design thinking workshop process. Students' thoughts are expressed in threedimensional forms while they empathise with other students through conversation. The materials used are sophisticated, empathy-oriented and provide easy access to rapid prototyping. Herein, we (1) explain the components of the developed toolkit, (2) provide a specific curriculum guide, (3) show the results of student experimentation with the toolkit in the prototyping stage of the design thinking workshop process and (4) demonstrate the toolkit's effectiveness by observing the students' creations and statements. Open coding and selective coding were used to analyse the themes that emerged from the interviews by students who participated in the workshop. We argue that the new prototyping toolkit can significantly promote students' creativity and design sensibility.

Epigenetic modifier lysine demethylase 3a (Kdm3a) specifically demethylates mono-and di-methylated ninth lysine of histone 3 and belongs to the Jumonji domain-containing group of demethylases. Kdm3a serves roles during various biological and pathophysiological processes, including spermatogenesis and metabolism, determination of sex, androgen receptor-mediated transcription and embryonic carcinoma cell differentiation. In the present study, physiological functions of Kdm3a were evaluated during embryogenesis of Xenopus laevis. Spatiotemporal expression pattern indicated that kdm3a exhibited its expression from early embryonic stages until tadpole stage, however considerable increase of kdm3a expression was observed during the neurula stage of Xenopus development. Depleting kdm3a using kdm3a antisense morpholino oligonucleotides induced anomalies, including head deformities, small-sized eyes and abnormal pigmentation. Whole-mount in situ hybridization results demonstrated that kdm3a knockdown was associated with defects in neural crest migration. Further, quantitative polymerase chain reaction revealed abnormal expression of neural markers in kdm3a morphants. RNA sequencing of kdm3a morphants indicated that kdm3a was implicated in mesoderm formation, cell adhesion and metabolic processes of embryonic development. In conclusion, the results of the present study indicated that Kdm3a may serve a role in neural development during Xenopus embryogenesis and may be targeted for treatment of developmental disorders. Further investigation is required to elucidate the molecular mechanism underlying the regulation of neural development by Kdm3a.