We used proteomics to detect regional differences in protein expression levels from mitochondrial fractions of control, ischemia-reperfusion (IR), and ischemic preconditioned (IPC) rabbit hearts. Using 2-DE, we identified 25 mitochondrial proteins that were differentially expressed in the IR heart compared with the control and IPC hearts. For three of the spots, the expression patterns were confirmed by Western blotting analysis. These proteins included 3-hydroxybutyrate dehydrogenase, prohibitin, 2-oxoglutarate dehydrogenase, adenosine triphosphate synthases, the reduced form of nicotinamide adenine dinucleotide (NADH) oxidoreductase, translation elongation factor, actin alpha, malate dehydrogenase, NADH dehydrogenase, pyruvate dehydrogenase and the voltage-dependent anion channel. Interestingly, most of these proteins are associated with the mitochondrial respiratory chain and energy metabolism. The successful use of multiple techniques, including 2-DE, MALDI-TOF-MS and Western blotting analysis demonstrates that proteomic analysis provides appropriate means for identifying cardiac markers for detection of ischemia-induced cardiac injury.

. Nitric oxidecGMP-protein kinase G signaling pathway induces anoxic preconditioning through activation of ATP-sensitive K ϩ channels in rat hearts. Am J Physiol Heart Circ Physiol 290: H1808 -H1817, 2006. First published December 9, 2005 doi:10.1152/ajpheart.00772.2005.-Nitric oxide (NO) plays an important role in anoxic preconditioning to protect the heart against ischemia-reperfusion injuries. The present work was performed to study better the NO-cGMP-protein kinase G (PKG) signaling pathway in the activation of both sarcolemmal and mitochondrial ATP-sensitive K ϩ (KATP) channels during anoxic preconditioning (APC) and final influence on reducing anoxia-reperfusion (A/R)-induced cardiac damage in rat hearts. The upstream regulating elements controlling NO-cGMP-PKG signal-induced KATP channel opening that leads to cardioprotection were investigated. The involvement of both inducible and endothelial NO synthases (iNOS and eNOS) in the progression of this signaling pathway was followed. Final cellular outcomes of ischemia-induced injury after different preconditioning in the form of lactate dehydrogenase release, DNA strand breaks, and malondialdehyde formation as indexes of cell injury and lipid peroxidation, respectively, were investigated. The lactate dehydrogenase and malondialdehyde values decreased in the groups that underwent preconditioning periods with specific mitochondrial KATP channels opener diazoxide (100 M), nonspecific mitochondrial KATP channels opener pinacidil (50 M), S-nitroso-Nacetylpenicillamine (SNAP, 300 M), or ␤-phenyl-1,N 2 -etheno-8-bromoguanosine-3Ј,5Ј-cyclicmonophosphorothioate, Sp-isomer (10 M) before the A/R period. Preconditioning with SNAP significantly reduced the DNA damage. The effect was blocked by glibenclamide (50 M), 5-hydroxydecanoate (100 M), N G -nitro-L-arginine methyl ester (200 M), and ␤-phenyl-1,N 2 -etheno-8-bromoguanosine-3Ј,5Ј-cyclic monophosphorothioate, Rp-isomer (1 M). The results suggest iNOS, rather than eNOS, as the major contributing NO synthase during APC treatment. Moreover, the PKG shows priority over NO as the upstream regulator of NO-cGMP-PKG signal-induced KATP channel opening that leads to cardioprotection during APC treatment.guanosine 3Ј,5Ј-cyclic monophosphate; adenosine 5Ј-triphosphate; oxidative damage ISCHEMIC PRECONDITIONING, in which short-term occlusion and reperfusion of a coronary artery are followed by long-term occlusion, can reduce subsequent ischemia-induced injury to the heart (42). Nitric oxide (NO), protein kinase G (PKG), and ATP-sensitive K ϩ (K ATP ) channels (both the sarcolemmal and mitochondrial subtypes) can mimic the effects of ischemic preconditioning in the heart, and mitochondrial K ATP channels appear to be the end effectors (19,20,25). The activation of these channels may improve the recovery of regional contractility of myocardium by shortening the duration of action potentials and by attenuating membrane depolarization, both of which would decrease myocardial contractility and reduce energy expenditure during ischem...

Origami, the ancient art of paper folding, has shown its potential as a versatile platform to design various reconfigurable structures. The designs of most origami-inspired architected materials rely on a periodic arrangement of identical unit cells repeated throughout the whole system. It is challenging to alter the arrangement once the design is fixed, which may limit the reconfigurable nature of origami-based structures. Inspired by phase transformations in natural materials, here we study origami tessellations that can transform between homogeneous configurations and highly heterogeneous configurations composed of different phases of origami unit cells. We find that extremely localized and reprogrammable heterogeneity can be achieved in our origami tessellation, which enables the control of mechanical stiffness and in-situ tunable locking behavior. To analyze this high reconfigurability and variable stiffness systematically, we employ Shannon information entropy. Our design and analysis strategy can pave the way for designing new types of transformable mechanical devices.

The use of an inconsistent speed limit determination method can cause low speed limit compliance. Therefore, we developed an objective methodology based on engineering judgment considering the traffic accident rate in road sections, the degree of roadside development, and the geometric characteristics of road sections in urban roads. The scope of this study is one-way roads with two or more lanes in cities, and appropriate sections were selected among all roads in Seoul. These roads have speed limits of the statutory maximum speed of 80 km/h or lower and are characterized by various speeds according to the function of the road, the roadside development, and traffic conditions. The optimal speed limits of urban roads were estimated by applying the characteristics of variables as adjustment factors based on the statutory maximum speed limit. As a result of investigating and testing various influence variables, the function of roads, the existence of median, the level of curbside parking, the number of roadside access points, and the number of traffic breaks were selected as optional variables that influence the operating speed. The speed limit of one-way roads with two or more lanes in Seoul was approximately 10 km/h lower than the current speed limit. The existing speed limits of the roads were applied uniformly considering only the functional road class. However, considering the road environment, the speed limit should be applied differently for each road. In the future, if the collection scope and real-time collection of road environment information can be determined, the GIS visualization of traffic safety information will be possible for all road sections and the safety of road users can be ensured.